IN VITRO AND IN VIVO TOXIC EFFECTS AND NEUROTOXICITY POTENTIALS OF DIMETHYL ISOPHTHALATE (DMIP)
Project no. 428 · Status: Kapanmış · 2017
Summary
Phthalates are esters of phthalic acid and are mainly used as plasticizers in a wide variety of products and applications which are added to plastics to increase their flexibility,transparency, durability, and longevity. They are mainly used in medical devices, cosmetics, emulsifying agents, toys and adhesives. Phthalates are not covalently bound to the plastic material and can easily leach into the environment. Dimethyl Isophthalate (DMIP) is used as a perfume fixative and as a plasticizer to make polyester resins. There is no information on DMIP toxicity. In this study, in vitro genotoxic and cytotoxic effects of DMIP were determined, ex-vivo assays were performed and then toxic and neurotoxic effects were investigated by in vivo acute and subacute toxicity tests. After single dose acute toxicity test LD50 value is calculated and with this value we start to 28 days subacute toxicity test with DMIP which is orally administered to mice 28 days, 5 days per week at the same time each day and at the end of the experiment biochemical and histological parameters were examined. In conclusion, with the toxicity data obtained from this study it is for the first time revealed that DMIP has not been found toxic in cytotoxicity and acute dose assays but it has been found to be a genotoxic substance that by AMES test results and a toxic substance in exvivo and subacute toxicity assays.