CONSTRUCTION OF IN VITRO DISEASE FREE NUCLEAR STOCKS OF CERTAIN POTATO GENOTYPES
Project no. 972 · Status: Kapanmış · 2016
Summary
In this study, potato clones cleaned from the following viruses PVS, PVX, PVY, PLRV, PVA and PMTV were obtained via meristem culture and they were increased in vitro. Seedlings were grown in the greenhouse and mini tubers obtained were tested in the seed beds. Based on the analysis of variance results the significant statistical differences among the clones were found. When the results of greenhouse experiment were evaluated: clone 101-1-3 (3,0) for single tuber number; clones 6/7-4 and 6/7-2-4 for single tuber weight and single plant yield had the high means as compare to the others. Based on the results of seed bed experiment the highest mini tuber number were obtained the clones of 6/7. Clone Hermes 1-4 for single tuber weight and Clone 122-1-2 for hill and plot yields were found to be higher than the other clones. Clones Atlantic 2-6; 2-7 and 2-8 (5,8 cm) had high tuber width and clones Hermes 1-4 and 2-1 (6,6 cm) were the highest means for tuber length. In conclusion, the virus free nuclear seed stocks of potato genotypes were obtained by using meristem culture and they were increased in the greenhouse and seed beds in order to obtain potato seed stocks in super elit-elit quality. Key Words: Potato (Solanum tuberosum L.), meristem culture, node culture, ELISA test, potato nuclear seed stocks