INVESTIGATION OF THE EFFECT OF ANTI-INFLAMMATORY AND BONE WAX AGENTS ON BRIDGETING IN THE EPIPHYSIS LINE IN A RAT MODEL WITH EXPERIMENTAL EPIPHYSIS İNJURY.

Project no. 21954 · Status: Kapanmış (Yayın bekleniyor) · 2020

Summary

Introduction: Growth plate injuries often lead to bone growth abnormalities, primarily due to the bone repair process in the injury areas. Before bone repair, an inflammatory response induced by the injury, which may play a role in regulating the repair process. This study investigates the roles of anti-inflammatory analgesics and bone wax agent in the damaged growth plate.Materials and Methods: An in vivo study was conducted on a total of 70 Wistar albino rats in seven different groups. Group 1 (n=10): sham group, Group 2 (n=10): injury group, Group 3 (n=10): indomethacin, Group 4 (n=10): bone wax, Group 5 (n=10): indomethacin and bone wax, Group 6 (n=10): local cortisone, and Group 7 (n=10): systemic cortisone. The study analyzed gene expressions, histological changes, immunohistochemistry, and the effects of blocking bone bridge formation through micro-CT. Measurements were taken on the third, seventh, and 28th days. Samples collected on the 28th day were first evaluated for bone volume using micro-CT and then underwent histopathological evaluation. PCR analysis was performed on the third day.Results: In the indomethacin and bone wax groups, most markers in the PCR study showed nonsignificant elevation. Local cortisone, on the other hand, showed a significant increase. In the histological study, bone density was found to be the lowest in the indomethacin and bone wax groups. Osteocalcin was lowest in the indomethacin group on the third day, while on the seventh day, it was lowest in the local cortisone group. TNF-α evaluation showed the lowest levels in the systemic cortisone group on the third and seventh days. The Runx2 marker on the third day was lowest in the local cortisone group, and on the seventh day, it was lowest in both the local cortisone and indomethacin groups. Although PCR, histology, and immunohistochemical results showed a decrease in inflammation after the anti-inflammatory applications, they failed to prove any protective effect against ossification. Micro-CT evaluation indicated a decrease in bone volume in all treatment groups, but there was no significant difference compared to the sham and control groups.Conclusion: Cortisone, indomethacin, and bone wax have been shown to reduce inflammation in epiphyseal damage. However, they have shown minimal impact on bone formation after inflammation. To further elucidate this issue, additional clinical research is needed to investigate the potential clinical benefits of using cortisone, indomethacin, and bone wax as adjuncts in epiphyseal ossification excision procedures.

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