Detection and Isolation of the Fraction of Staphylococcus Species Exhibiting In Vitro Antimicrobial Activity
Project no. 21461 · Status: Kapanmış (Yayın bekleniyor) · 2020
Summary
<p>Tuberculosis is one of the most important causes of preventable adult deaths worldwide. In addition, the incidence of non-tuberculous mycobacterial infections is constantly increasing in the world. Because many antibiotics have lost their efficacy, researchers have had to identify new types of therapeutic molecules. Bacteriocins inhibit the growing of prokaryotes and could potentially be effective against antibiotic-resistant bacterial strains. In this study, it was aimed to identify the substance(s) produced by a <em>Staphylococcus epidermidis</em> strain and showing antimycobacterial activity in vitro. The <em>in vitro</em> antimycobacterial effect was observed in different staphylococcal strains in the study, suggesting that the synthesis of the antimycobacterial molecule is encoded by chromosomal genes, it is probably common in staphylococcal strains and species, and this substance may not be a classical bacteriocin. In addition, the fact that the antimycobacterial effect is parallel to the increase in the expression of the genes responsible for lipid metabolism in bacteria suggests that the substance/s with antimycobacterial effect may be effective on fatty acid synthesis or that the increase in the expression of the genes responsible for lipid metabolism may induce bacteriocin production in bacteria by a kind of quorum sensing (QS) mechanism. In addition, the absence of ebh found in <em>Staphylococcus aureus</em>, <em>S. epidermidis</em> and other staphylococcal species in <em>Staphylococcus intermedius</em>, which does not show antimycobacterial activity in vitro, and the increase in its expression in parallel with the antimycobacterial activity suggested that the protein encoded by ebh may have a role in this effect.</p>