Effects of omega-3 fatty acids on proinflammatory cytokines in an experimental model of sepsis
Project no. 29 · Status: Kapanmış · 2016
Summary
In our study, we aimed to investigate the effects of omega-3 fatty acids on the level of proinflammatory cytokines (IL-1, IL-6, TNF?) and survival in a sepsis rat model. In the study, 40 albino Wistar adult rats weighing 300 gr were divided into 3 groups; Group 1: Sham Group: (n = 10) (Tactile stimulation of the intestine and cecum after laparotomy), Group 2: Control Group: (n = 15) (1 ml / kg saline was given intraperitoneally for 1 week before sepsis model with cecal perforation), Group 3: Omega-3 Group: (n = 15) (1 ml / kg omega-3 fatty acid was given intraperitoneally for 1 week before sepsis model with cecal perforation). IL-1, IL-6 and TNF-? levels were measured at the beginning of the study (basal value) and at 3rd hour in all groups. 24 hour survival rate was 60% in the omega-3 group and 13.3% in the control group (p = 0.008). In the omega-3 group, serum IL-1, IL-6 and TNF-? levels were significantly lower than the control group at the 3rd hour (p <0.001). To conclude, we determined the positive effects of omega-3 fatty acids on proinflammatory cytokine levels, immune response development and survival in experimental sepsis rat model which is considered to be related to the anti-inflammatory and immunmodulatuar properties of omega-3 fatty acids.