Applications, Characterization and Integration of Biological Molecules with Nanostructured Materials
Project no. 485 · Status: Kapanmış · 2018
Summary
Producing biomaterials in nanoscale architecture is one of the most popular topic as biological and nanotechnologic applications particularly relies on biocompatibility and biodegradability. Among other biomaterials, carbohydrate- based polymers satisfy the demand in terms of availability, cost-efficiency and physicochemical properties. Electrospinning is a promising and simple technique to produce carbohydrate-based nanofibers. In this study, one of the cellulose derivative cellulose acetate (CA) was blended with chitosan (CS) and CA-CS nanofibers were synthesized via electrospinning technique. Different solvent systems and CA-CS polymer ratio were examined and process parameters were optimized to obtain the ultrafine and bead-free CA-CS nanofibers. Scanning electron microscopy (SEM) was performed to characterize the surface morphology of CA-CS nanofibers. Then, to test the CA-CS nanofibers as an immobilization material; glucose oxidase (GOx) was immobilized on CA-CS coated GCE by covalent modification to design biosensor platform. CV, DPV, EIS measurements were carried out to prove the surface modification and CA- CS/GOx biosensor was tested for glucose detection by amperometric technique. Limit of detection (LOD) and linear response for glucose of CA-CS/GOx biosensor was found 14 µM and 5 µM-0,75 mM respectively. Finally, CA- CS/GOx platform was investigated to detect glucose in synthetic samples.