Evaluation of the Effects of Sweet Almond Oil on UVA-Induced Damage in Dermal Fibroblasts
Project no. 32400 · Status: Kapanmış · 2024
Summary
<p>Solar radiation, through its ultraviolet (UV) components, causes various undesirable effects on human skin, including photoaging, pigmentation disorders, and the development of skin cancer. In this study, a cell culture–based approach will be employed, including cytotoxicity analysis and UVA irradiation, to determine the mRNA expression levels of target genes involved in type I and type III collagen synthesis. Dermal fibroblast cell cultures will be exposed to UVA radiation, which is primarily responsible for photoaging. Sweet almond oil will be applied to one group of cell cultures. By evaluating dermal fibroblast number, cellular configuration, and collagen synthesis before and after UVA exposure, the potential effects of almond oil on photoaging will be investigated.</p><p>As a result of this project, it is anticipated that the bioactive components of sweet almond oil (such as α-tocopherol, polyphenols, etc.) will exhibit photoprotective effects against pre- and post-UV–induced damage in cell culture models and enhance collagen synthesis. The limited number of studies in the literature investigating plant-derived products in the context of UVA irradiation is noteworthy. Therefore, this project will specifically focus on the UVA wavelength, which is responsible for the dermal effects of photoaging. In addition, unlike other studies, the photoprotective effects of almond oil will be investigated in human dermal fibroblast cells rather than animal models, and the effects of almond oil on collagen synthesis and cell number before and after UV exposure will be evaluated along with cytotoxicity analysis. Determining the effective concentration ranges will pave the way for the potential use of almond oil as an effective ingredient in sun-protective dermocosmetic products.</p>