Determination of Anomura (Decapoda, Crustacea) Species of Karaburun Peninsula by DNA Barcoding

Project no. 31848 · Status: Kapanmış (Yayın bekleniyor) · 2023

Summary

<p>This&nbsp;study&nbsp;aims&nbsp;to&nbsp;investigate&nbsp;the&nbsp;species&nbsp;diversity&nbsp;of&nbsp;the&nbsp;Anomura&nbsp;taxon&nbsp;in&nbsp;the&nbsp;Karaburun&nbsp;Peninsula&nbsp;by&nbsp;supporting&nbsp;morphology-based&nbsp;identifications&nbsp;with&nbsp;genetic&nbsp;(DNA)&nbsp;barcoding.&nbsp;In&nbsp;addition,&nbsp;the&nbsp;study&nbsp;seeks&nbsp;to&nbsp;establish&nbsp;a&nbsp;baseline&nbsp;dataset&nbsp;for&nbsp;Anomura&nbsp;in&nbsp;the&nbsp;region&nbsp;and&nbsp;to&nbsp;contribute&nbsp;the&nbsp;obtained&nbsp;COI&nbsp;(cytochrome&nbsp;c&nbsp;oxidase&nbsp;subunit&nbsp;I)&nbsp;nucleotide&nbsp;sequences&nbsp;to&nbsp;international&nbsp;databases&nbsp;such&nbsp;as&nbsp;BOLD&nbsp;and&nbsp;NCBI&nbsp;GenBank,&nbsp;thereby&nbsp;providing&nbsp;the&nbsp;first&nbsp;genetic&nbsp;records&nbsp;for&nbsp;this&nbsp;taxon&nbsp;from&nbsp;Türkiye.&nbsp;This&nbsp;dataset&nbsp;is&nbsp;intended&nbsp;to&nbsp;serve&nbsp;as&nbsp;a&nbsp;reference&nbsp;for&nbsp;future&nbsp;biodiversity,&nbsp;taxonomic&nbsp;and&nbsp;phylogenetic&nbsp;studies.</p><p>The&nbsp;Karaburun&nbsp;Peninsula,&nbsp;designated&nbsp;as&nbsp;a&nbsp;Special&nbsp;Environmental&nbsp;Protection&nbsp;Area&nbsp;(SEPA)&nbsp;in&nbsp;2019,&nbsp;represents&nbsp;an&nbsp;important&nbsp;natural&nbsp;laboratory&nbsp;for&nbsp;assessing&nbsp;the&nbsp;effects&nbsp;of&nbsp;conservation&nbsp;measures&nbsp;on&nbsp;biological&nbsp;diversity.&nbsp;Accordingly,&nbsp;this&nbsp;study&nbsp;integrates&nbsp;traditional&nbsp;morphological&nbsp;identification&nbsp;with&nbsp;molecular&nbsp;methods,&nbsp;including&nbsp;DNA&nbsp;extraction,&nbsp;amplification&nbsp;and&nbsp;sequencing&nbsp;of&nbsp;specimens&nbsp;collected&nbsp;from&nbsp;different&nbsp;habitats&nbsp;in&nbsp;the&nbsp;region.&nbsp;The&nbsp;resulting&nbsp;DNA&nbsp;sequences&nbsp;will&nbsp;be&nbsp;compared&nbsp;with&nbsp;barcode&nbsp;records&nbsp;from&nbsp;global&nbsp;databases,&nbsp;and&nbsp;the&nbsp;outcomes&nbsp;will&nbsp;be&nbsp;evaluated&nbsp;in&nbsp;conjunction&nbsp;with&nbsp;morphological&nbsp;re-examinations.</p><p>Furthermore,&nbsp;phylogenetic&nbsp;analyses&nbsp;will&nbsp;be&nbsp;used&nbsp;to&nbsp;infer&nbsp;evolutionary&nbsp;relationships&nbsp;and&nbsp;genetic&nbsp;affinities&nbsp;among&nbsp;the&nbsp;detected&nbsp;species.&nbsp;By&nbsp;generating&nbsp;the&nbsp;first&nbsp;genetic&nbsp;barcodes&nbsp;for&nbsp;Anomura&nbsp;species&nbsp;from&nbsp;the&nbsp;Karaburun&nbsp;Peninsula,&nbsp;this&nbsp;study&nbsp;provides&nbsp;an&nbsp;original&nbsp;contribution&nbsp;to&nbsp;regional&nbsp;biodiversity&nbsp;knowledge&nbsp;while&nbsp;also&nbsp;supporting&nbsp;the&nbsp;evaluation&nbsp;of&nbsp;conservation&nbsp;effectiveness&nbsp;and&nbsp;enriching&nbsp;international&nbsp;genetic&nbsp;reference&nbsp;libraries.</p>

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