ENVIRONMENTAL POLLUTANT LEVELS IN HUMAN STOMACH AND KIDNEY CANCEROUS TISSUES, PHENOTYPIC AND GENOTYPIC PROPERTIES OF DRUG METABOLIZING ENZYMES
Project no. 392 · Status: Kapanmış · 2014
Summary
In this project, the possible role of POP derivatives in human stomach and kidney cancers was investigated. POP levels in blood, tumor and adipose tissue samples taken from patients who underwent surgery for both cancer diagnoses, and in blood, control tissue and omentum samples from patients who underwent gastric reduction surgery. Also DNA and protein macromolecular damage parameters (8-OHdG, o,o'-dityrosine, chlorotyrosine and nitrotyrosine, respectively) were determined in urine samples. Genetic variations of cytochrome P450 1A1 (CYP1A1), which is involved in the biotransformation of POP derivatives, 3 different glutathione S-transferase (GST) isozymes, and human 8-oxoguanine glycosylase-1 (hOGG1) enzyme, which is involved in DNA repair, were analyzed in Ankara University within the scope of the project collaboration. The levels of some POP derivatives were determined to be significantly higher in the blood and tissue samples of gastric and kidney cancer groups compared to the controls. In addition, significant correlations were found between blood and tissue POP levels in gastric and kidney groups. o,o?-dityrosine was detected at a higher level in the urine samples of the stomach control group, while 8-OHdG was determined at a higher levels in the urine samples of the kidney malignant tumor group. It has been determined that variation in genes encoding GSTP1 and CYP1A1 enzymes may be a risk factor in gastric cancer, and variation in genes encoding GSTT1 enzyme may be a risk factor in the development and/or formation of kidney malignant tumors. CYP1A activity of gastric and kidney cancerous tissues was determined at similar levels compared to control tissues, but significant correlations were found between POP levels and CYP1A activity of the tissues.