PROSTATE CANCER STEM CELL ISOLATION
Project no. 50 · Status: Kapanmış · 2016
Summary
Adhesion molecules have a great importance in the attachment of cells to each other and surrounding tissue. Cells in adhesive cell cultures accumulate together and tend to form clusters and tissues. This tendency plays a major role in determining the fate of the tissue, depending on whether the cell is healthy or malignant. Healthy cells are effective in the formation of healthy tissue, while malignant cells are effective in the growth, invasion, and even micrometastasis of the tumor mass. Cancer stem cell model is among the topics discussed in recent years. Recent studies have shown that cancer cells are the primary target for understanding tumor biology and determining treatment methods. This project aimed to investigate the changes in the adhesion molecules necessary for the development of cancer stem cells during adherence to each other and for their ability to maintain their metastasis and invasion abilities. Other cancer cell groups with cancer stem cells will be separated by CD133 surface marker. These two groups of cells (stem cell - non-stem cell cancer cells) will be reproduced in monolayer cell cultures and in the 3D spheroid model. The aim of this study was to show whether surface markers and ultrastructures of prostate cancer cells have changed in monolayer cultures and the spheroid formation changes when tumor tissue gains 3D structure. At the end of this study, a change in cancer stem cell adhesion molecules and microstructure may provide a new step in determining new targets. Key words: prostate cancer cells, stem cell, adhesion molecules, spheroid formation