EFFECTS OF ENCAPSULATED POSTBIOTIC AND INULIN ADDITION TO BROILER FEEDS ON PERFORMANCE, CARCASS, MEAT QUALITY, GUT, BLOOD AND IMMUNE PARAMETERS, DIGESTIBILITY OF NUTRIENT AND SOME HORMONE MRNA EXPRESSION

Project no. 23783 · Status: Kapanmış · 2022

Summary

This study examined the effects of supplementation of postbiotics produced from different probiotic microorganisms (Lactobacillus plantarum, Bacillus subtilis, and Enterecoccus faecium) on performance, carcass, meat quality, digestibility of nutrients, intestinal parameters, mRNA expression of some hormones, and blood parameters when they were added to mixed feed for broilers in encapsulated form alone or in combination with inulin. Day-old 588 male broiler chicks (of Ross-308 genotype) were randomly separated into groups as 7 treatments and 6 repetitions (repetition: 14). The experiment lasted for 42 days and consisted of a control group which was given basal mixed feed without any growth promoting additives and broiler groups which were fed with supplementations of postbiotics produced from different probiotic microorganisms alone (0.3%) or in combination with inulin (1%). On the 17th-21st and 38th-42nd days of the experiment, 6 repetitions from each treatment were selected, fed in individual cages. In light of the data obtained from the experiment, it was established that supplementation of postbiotics alone or in combination with inülin had an enhancing effect on body weight and body weight gain. There was no effect on feed intake between days 0-21, however it was increased between days 22-42 and 0-42. Feed conversion ratio was improved between days 0-21, but it had not seen a significant effect between days 22-42 and 0-42. The best growth performance was detected among animals which were fed with postbiotics produced from Bacillus subtilis, alone or in combination with inulin. It was determined that postbiotics supplementation alone or in combination with inulin in broiler chickens enhanced carcass, breast, and thigh weight and yield, but it reduced abdominal fat ratio. As for digestive organs, proventriculus and gizzard weights were increased along with duodenum and jejenum+ileum weights and relative weights. However, large intestine and cecum weights and relative weights have not been affected. Among other internal organs, no effect was observed on weights of heart and spleen or relative weights of heart, spleen, liver, and pancreas, whereas there was an increase in liver and pancreas weights and bursa fabricius weight and relative weights. It was observed that the supplementation of postbiotics in encapsulated form alone or in combination with inulin had no effect on nutrients digestibility in broiler chickens. Furthermore, it had no effect on nutrient contents and meat quality parameters such as colour, water holding capacity, thawing and cooking loss, but it enhanced malondialdehyde range. Villus height, villus width, and crypt depth of duodenum, jejunum, and ileum were found to be enhanced. Although total mucosal thickness was increased in duodenum, there was no significant effect on it in jejunum and ileum. Acetic acid, propionic acid, and butyric acid levels and lactic acid bacteria concentrations in cecum were increased, but Escherichia coli level was reduced. Supplementation of postbiotics alone or in combination with inulin increased the levels of insulin-like growth factor and mRNA expression of growth hormone. Concentrations of some liver enzymes, cholesterol, glyceride, and total oxidant activity in blood serum has seen a reduction, meanwhile total antioxidant status, immunoglobulin A and M levels were increased. There was no effect on the levels of total protein and glucose in blood serum. In conclusion, postbiotics produced from Lactobacillus plantarum, Bacillus subtilis, and Enterecoccus faecium in encapsulated form alone (0.3%) or in combination with inulin (1%) had positive effects on performance, carcass, microbiota, and immune parameters in male broilers. Nevertheless, it had no significant effect on meat quality (except MDA) and digestibility of nutrients. The use of postbiotics (especially those produced from Bacillus subtilis) alone or in combination with inulin can be recommended as an alternative feed additive to antibiotics for broiler chickens.Keywords: Bacillus subtilis, Enterococcus faecium, Lactobacillus plantarum, postbiotics, inulin, encapsulated form, broiler, performance, carcass, microbiota, immunity.

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