Investigation of Toxic Effects of Carbofuran and Fenamiphos on Daphnia magna (Crustacea, Cladocera)
Project no. 23180 · Status: Kapanmış · 2021
Summary
<p><em>Daphnia</em>, known as the water flea, is a small crustacean that lives in fresh water such as lakes, ponds, and streams. The water flea serves as a prominent food source for fish and other aquatic organisms. <em>Daphnia</em> is an excellent organism used in laboratory experiments because it is sensitive to changes in the water content and daphnids are simple and cheap to grow in aquariums. They mature in a short time, so it does not take long to grow a culture of test organisms. Therefore, in this study, the effects of Carbofuran on the heart rate and egg production of <em>D. magna</em> individuals were evaluated. Due to the deterioration of Fenamiphos, the effect of the chemical could not be examined. Four groups were formed as control and treatment groups and each group included 10 individuals. Water fleas in the treatment groups were exposed to different concentrations of Carbofuran (3 µg/L, 6 µg/L, 12 µg/L). All experiments were performed in triplicate. Heart rate variability was examined for 96 hours, and heart rate was calculated using a video camera. Additionally, the number of eggs per individual in each experimental group was recorded for 21 days to evaluate the effects of the pesticide on egg production. An increase in heart rate was observed due to exposure to Carbofuran, depending on the degree of concentration. Carbofuran exposure adversely affected egg production. A decrease was observed in the number of eggs per individual depending on the concentration levels. Additionally, compared to the control group, the day of first egg production were considerably delayed in the high and medium-dose groups.</p>