Investigating the Anaphase Promoting Complex Activity and HN1 interaction
Project no. 21986 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
Prostate cancer is one of the most common cancers for men worldwide, with advanced forms showing clustered centrosomes. Hematologically and neurologically expressed 1 (HN1) belongs to a small family of poorly understood genes that are evolutionarily conserved among vertebrate species. The TCGA PRAD database demonstrates the putative role of HN1 in centrosome-related processes in prostate cancer. Dysregulation of the Anaphase-progressive complex/cyclosome (APC/C) pathway, an important E3 Ligase in cells, causes mitotic catastrophe, chromosomal instability, and uncontrolled division that can result in cancer. In our previous studies, we found the association of HN1 with mitotic regulators. We found that mitotic damage such as chromosome folds (tetraploidy), micronuclei formations, anaphase arrest, cytokinase damage occurred in cells when HN1 was silenced with siHN1 in support, and monopolar spindle formation occurred as a result of multiple microtubule formation. Coactivators of APC/C and other unidentified protein associations continue to be investigated. The functions of APC/C that are responsible for microtubule synthesis and mechanisms indicating cell polarization need to be clarified. It was thought that an early cdh1 activation in the presence of HN1 might cause endomitosis, causing chromosome number folding and disruption of centrosome separation, and it was aimed to investigate the deficiency in the literature. Data from the project identified the role of HN1 in cell cycle and centrosome regulation in progressive prostate cells. Since the data obtained is related to cell cycle and mitosis control, it can be published in high-impact factor journals. In addition, this project contributed to the training of well-equipped graduate students by informing them about cancer biology.