ISOLATION and STRUCTURALLY IDENTIFICATION of SAPONINS from ENDEMIC YILDIZ PELEMIRI PLANT
Project no. 21173 · Status: Kapanmış · 2019
Summary
<p>In this study, it was aimed to isolate, purify, determine the structure and the biological activity potential of natural saponins from <em>Cephalaria speciosa</em> (Caprifoliaceae) plant. Phytochemical studies on the aerial parts of the plant were resulted with total of 11 saponins 4 of which were revealed as new compounds with 3 new prosapogenins. Total of 6 known saponins namely, macranthoidin A (<strong>5</strong>), elmalienoside A (<strong>6</strong>), dipsacoside B (<strong>7</strong>), decaisoside E (<strong>8</strong>), scoposide A (<strong>9</strong>), 3-O-<em>β</em>-D-glucopyranosyl-(1→3)-<em>α</em>-L-rhamnopyranosyl-(1→2)-<em>α</em>-L-arabino-pyranosyl oleanolic acid 28-<em>β</em>-D-glucopyranosyl-(1→6)-<em>β</em>-D-glucopyranosyl ester (<strong>10</strong>), were isolated. Various chromatographic (CC, VLC, TLC, MPLC) and chemical methods were used during purification and isolation studies. Advanced spectroscopic methods (FTIR, 1D- and 2D-NMR, HR-MS) were used to determine the exact structures of the pure compounds. The cytotoxic activities of the novel saponins and their basic hydrolysis products, prosapogenins were investigated for A549, CCD34-Lu, MDA-MB-231, PC-3, U87MG, HeLa, HepG-2 cell lines. Compound <strong>1</strong>, prosapogenins <strong>2 </strong>and <strong>4 </strong>showed significant cytotoxicity.</p>