Effects of Calcium Sulphate (CaSO4) and Potassium Chloride (KCl) on Reproduction and Heart Rate of Daphnia magna
Project no. 22478 · Status: Kapanmış · 2020
Summary
In fish farming, Daphnia magna is used as a live food especially during the development of some fish species (carp, sturgeon, catfish, freshwater bream, perch etc.). This species can reproduce rapidly in suitable environment and temperature (15-22 °C). D. magna reproduction in the laboratory is extremely simple and inexpensive. Therefore, D. magna is a frequently used experimental animal in laboratory studies. The effects of two different compounds (KCl and CaSO4) on reproduction and heart rate of D. magna were examined in this study. Ten individuals were used in each of control and treatment groups. D. magna were exposed to different concentration of both KCl and CaSO4 (37.5, 75, 150 mg/L). All experimental samples were made in triplicate. Changes in heart rate were examined for 96 h and the number of heart beats per minute was counted by using a video camera. Egg production in all experimental groups were recorded for 21 days. As a result of the exposure to CaSO4, an increase in heart rate and egg reproduction dependently of CaSO4 concentration gradient was examined. However, exposure to KCl caused a decrease in egg production in medium and high dose groups. Additionally, although heart rate increased for two consecutive days after exposure to KCl, a decrease in heart rate was observed during the last 48 h.