METHOTREXATE LOADED SILICA NANOPARTICLES: SYNTHESIS, RADIOLABELING AND IN VITRO EVALUATION
Project no. 213 · Status: Kapanmış · 2017
Summary
Recently, the advantages provided by nanoparticles (NPs) in the transport of therapeutic and target-specific drugs have received increasing attention by scientists and the pharmaceutical industry. Anticancer drugs have some side effect effects such as healthy tissue toxicity, low stability, tumor cell resistance to drugs. In order to overcome these side effects, many different drug delivery systems such as liposomes, polymers, micelles, antibodies have been investigated. Among these carrier systems, mesoporous silica nanoparticles have suitable properties. In this study mesoporus silica nanoparticles (MSN) loaded with methotrexate (MTX), an anticancer drug used in breast cancer treatment, were synthesized. The particle size, morphology and drug loading capacity of the synthesized nanoparticles were analyzed and optimum conditions were determined. Characterization of nanoparticles was performed by Dynamic Light Scattering (DLS), Scanning electron microscope (SEM). According to the DLS analysis, the MSN was 126 ± 10 nm in size and the polydispersity index (PdI) was 0.24. According to the SEM analysis, the synthesized nanoparticles are monodisperse and spherical morphology in the range of 50-65 nm. Then, MTX loaded MSN carrier system was radiolabeled with Tc-99m with a radiolabeling yield of 92.20 ± 0.8%. After quality control studies, cytotoxicity and uptake studies on cancer and healthy cell lines of radiolabeled MSN carrier system were performed in vitro using estrogen positive (ER +) MCF-7 and estrogen negative (ER-) A549 cells. Drug loaded MSN particles were found to exhibit higher uptake in MCF-7 cells than A549 cells. It is thought that the optimized drug delivery system obtained at the end of the project will be evaluated for further studies to be used in drug delivery system formulations of anticancer drugs.