Investigation of thyroid functions and thyroid histological structure in an experimental sepsis model, specific to necroptosis
Project no. 20127 · Status: Kapanmış (Yayın bekleniyor) · 2019
Summary
<p><strong>Abstract</strong></p><p><strong>Introduction and aim:</strong> Thyroid hormone levels are known to decrease in sepsis, and this change is caused by non-thyroidal sickness syndrome (NTIS). The mechanism of NTIS is not fully elucidated. It has been suggested that the decrease in thyroid hormone levels in sepsis is due to histopathological changes in the thyroid tissue and NTIS, and the structural change in the thyroid tissue develops in the direction of necroptosis. In our study, it was aimed to i) evaluate serum thyroid function (T3, T4, and TSH) levels by biochemical analysis, ii) immunohistopathological evaluation of changes in the thyroid tissue, and iii) elucidate the mechanism of necroptosis in the experimentally created rat sepsis model.</p><p><strong>Materials and Methods:</strong> In the study, 44 adult male rats of <em>Wistar Albino</em> species weighing approximately 300 g were used. While the control and Sham groups each had 8 rats, the Sepsis groups were designed to contain 10 rats due to the possibility of death. The cecal ligation and perforation (CLP) model was used to create sepsis under general anesthesia. Thyroid tissue was used for histological and immunohistochemical examinations with routine follow-up procedures. Thyroid hormone analyses (T3, T4, and TSH) were carried out using the ELISA technique.</p><p><strong>Results:</strong> The obtained results showed that the histological structure of the thyroid tissue was impaired and there were significant degenerations in the follicle structures. Significant increases were detected in the immunoreactivity of RIPK1, RIP3, and MLKL, which are biomarkers of the necroptotic pathway, due to sepsis. ELISA analyses displayed a decrease in T3 and T4 levels in the sepsis groups.</p><p><strong>Conclusion:</strong> When the biochemical, morphological, and immunohistochemical results in our study were evaluated, it was observed that there was a decrease in thyroid hormone levels in the early period of sepsis, cell death in the thyroid tissue, and the necroptosis pathway due to sepsis was activated. It was concluded that the obtained data can guide the management of sepsis and that there is a need for studies in which immunohistopathological/biochemical data will be evaluated.</p><p><strong>Keywords:</strong> Sepsis, Thyroid, Necroptosis</p><p><br></p><p><br></p><p><br></p>