Investigation of the Effect of Resveratrol Against Paraquat-Induced Damage in Human Prostate Cancer Cell Lines
Project no. 20064 · Status: Kapanmış (Yayın bekleniyor) · 2018
Summary
<p>Resveratrol (RES) (trans-3, 5,-4'-trihydroxystilebene) is a multifunctional plant chemical found in grapes and mulberries. Resveratrol research has revealed that it can suppress the development of cancer cells and free radicals. Resveratrol is particularly efficient in inhibiting cell growth and inducing cell death in malignant cell lines while protecting normal cells and tissues. The goal of this work is to look into the effect of resveratrol in protecting healthy prostate cells from oxidative stress caused by paraquat induction, as well as to reveal its action on the Nrf2 pathway. It also aims to assess the response of resveratrol in prostate cancer cells to healthy prostate cells.</p><p>Resveratrol inhibits oxidative phosphorylation at the mitochondrial complex I and II levels, increasing the production of reactive oxygen species and, as a result, promoting the expression of Nrf2-driven antioxidant/detoxifying genes. The cytoprotective effect of Nrf2 downstream genes/proteins encouraged us to study if and how resveratrol promotes resistance to pro-oxidant damage in PC-3 and DU-145 prostate cancer cells.</p><p>We discovered that resveratrol pretreatment had an additive/synergistic impact on H2O2/docetaxel-induced cytotoxicity but did not increase paraquat/hydroquinone-induced cytotoxicity. Cytotoxicity, CAT, SOD, GSH-Px, Xanthine-Ox, MPO, MDA, Western Blot, and Nrf2 tests were done in healthy cell lines RWPE-1 and PC-3, as well as Du-145 cell lines. Finally, the adaptive response to Resvaratrole implies that activating Nrf2 may help cancer cells survive in an unfavorable environment.</p>