Comparison of the effect of autogenous tooth bone graft on bone repair and regeneration with different bone grafts: an in vitro study
Project no. 22407 · Status: Kapanmış (Yayın bekleniyor) · 2020
Summary
<p><strong>Aim: </strong>The aim of this project was to investigate the effect of autogenous tooth bone graft on proliferation, migration, attachment and mineralized tissue formation of human bone epithelial osteosarcoma (Saos-2), human gingival fibroblast (hGF), human peripheral blood monocyte cells (THP-1) and human periodontal ligament fibroblast cells (hPdLF), as an <em>in vitro</em>.</p><p><strong>Materials and Methods:</strong> Cell viability for autogenous tooth bone graft, allograft, xenograft and synthetic bone grafts were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) analysis. Alizarin red staining and osteocalcin activity test were used to assess bone calcification. Cell adhesion and morphology were examined with scanning electron microscopy (SEM). Cell proliferation and migration outcomes at 24, 48 and 72 hours was tested by analysis of variance (ANOVA) and Tukey's test was used for post-hoc comparisons.</p><p><strong>Results: </strong>The highest proliferation rates for Saos-2, hGF, THP-1 and hPdLF cells at 72 hours were observed in autogenous tooth bone graft. There was no statistically significant difference between the groups for cell migration. Calcification values and osteocalcin activities obtained by alizarin red staining in Saos-2 cells were higher in autogenous tooth bone graft, when compared to allograft, xenograft and synthetic bone graft.<strong> </strong>SEM analysis revealed that cells attached, spread, and showed similar morphological features to autogenous tooth bone graft, similar to allograft, xenograft, and synthetic bone graft.</p><p><strong>Conclusion: </strong>It is thought that<strong> </strong>autogenous tooth bone graft can be used as an alternative augmentation material by its structural properties and biocompatibility.</p>