Investigation of Antimicrobial and Antibiofilm Effects of Organic Antibiotics Produced From Cave Bacteria
Project no. 23843 · Status: Kapanmış · 2022
Summary
<p>Due to the widespread and excessive misuse of antibiotics globally and in our country, there has been a rapid increase in antibiotic resistance. Therefore, there is a need for innovative approaches, especially in the diagnosis and treatment of infections caused by biofilm-forming microorganisms. One of these innovative approaches, which also constitutes the topic of our doctoral thesis project, is the "production of organic antibiotics from cave bacteria as an alternative to contemporary synthetic antibiotics."</p><p>In the proposed study, bacterial samples will be collected from the "Morca" system cave in the Anamur district of Mersin, which has not been fully explored yet. Organic antibiotics produced by cave bacteria will be isolated from the bacterial culture medium. The antimicrobial effects of organic antibiotics will be determined by using the minimal microdilution method on pathogenic microorganisms, assessing inhibitor concentrations and bacterial killing power over time.</p><p>Some radionuclides used in nuclear medicine can be combined with molecules that selectively target cancerous or infected tissues (radioisotopic agents), directing them to these tissues. In this thesis, the organic antibiotics with confirmed antimicrobial activity will be combined with 131I (Iodine-131) radioisotope used for diagnostic and therapeutic purposes in Nuclear Medicine (with 364 keV γ E and t1/2= 8 days), giving them the property of a radioisotopic agent.</p><p>The unique value of the project lies in "the production of organic antibiotics from bacterial samples in a cave in Turkey that has not been fully explored, and the potential use of their radio-labeled derivatives in the diagnosis of infections in Nuclear Medicine." While there are few studies on the isolation of cave bacteria in the literature, no study has been found on radio-labeled organic antibiotics. The thesis project, focused on the synthesis of pre-clinical organic antibiotics and radio-labeled organic antibiotics, is expected to make a significant contribution to scientific research on the diagnosis of infectious diseases and the development of next-generation antibiotics targeting biofilm disruption.</p>