Comparative transcriptome analysis of resistance genes against Fusarium oxysporum f sp ciceris race 0 in wild (C reticulatum) and cultivated (C arietinum) chickpeas with RNA-Seq.
Project no. 23253 · Status: Kapanmış (Yayın bekleniyor) · 2022
Summary
<p>Chickpea (<em>Cicer arietinum</em> L.), belonging to the <em>Leguminosae</em> family and from the <em>Papilionoideae</em> subfamily, is a plant of high economic value with a genome size of 732 Mbp, characterized by self-pollination and diploid (2n=2x=16) chromosomes. Fusarium wilt stands out as one of the most significant biotic stress factors in chickpea cultivation, often resulting in 100% crop losses in years with suitable climatic conditions. Despite the application of cultural precautions, the development of resistant varieties through breeding is regarded as the most valid approach in combating this disease. One of the methods employed for gene detection involves the expression of relevant genes relative to control conditions. Within the framework of the differential gene expression analysis, mRNA from sensitive and resistant chickpea genotypes (<em>C. arietinum</em> (cultivated) and <em>C. reticulatum </em>(wild)) was isolated and converted into cDNA following inoculation with Foc0. The obtained cDNAs were sequenced using NGS technology to investigate differences in mRNA expression. Relative to controls of sensitive and resistant genotypes, it was found that 5,297, 2,079, 2,486, and 535 genes were up-regulated, while 4,147, 1,917, 2,490, and 166 genes were down-regulated, respectively. Differential gene expression analysis revealed a statistically significant total of 893 DEGs, with 683 DEGs identified in sensitive genotypes and 210 DEGs in resistant genotypes. Additionally, functional annotation, GO enrichment, and pathway analysis of the expressed genes were conducted. Upon treatment of chickpea genotypes with Foc0, it was observed that the expression levels of genes associated with cell wall integrity, peroxidase activity, response to oxidative stress, extracellular region, catabolic processes, reactive oxygen species, apoplast, and ion binding significantly increased across all genotypes. In conclusion, Fusarium wilt resistance genes in chickpea were directly identified based on mRNA expression within the scope of this project.</p>