KOYUNLARDA,CMAH GENİNİN NEU5GC SİALİK ASİDİYLE İLİŞKİSİNİN ARAŞTIRILMASI

Proje no. 668 · Durum: Kapanmış · 2017

Özet

Meat is a food source rich in nutrients that are essential for healthy human growth and development. Despite the fact that meat eating is now linked to disease including cancer, cardiovascular disease and diabetes, meat has played a crucial role in human evolution. As the world's population grows, the demand for meat and animal products which are essential for human health and nourishment is also increasing. In Turkey, where the population is continuously growing, it is critical to consume red meat and meat products in order to maintain a healthy and balanced diet.Red meat is one of the top ten factors linked to the occurrence and progression of carcinomas in all populations, according to a report issued by the World Cancer Research Foundation. Inflammation caused by the interaction of the N-glycolylneuraminic acid (Neu5Gc) molecule, a non-human sialic acid, with circulating anti-Neu5Gc antibodies has been proposed as a possible link between cancer and meat eating. As a result, it has been suggested that diets low in Neu5Gc are beneficial in lowering cancer risk.In the light of this information, the aim of this study was to analyze the CMAH mRNA sequence involved in Neu5Gc synthesis in Kıvırcık and Karacabey Merino sheep breeds reared in Turkey, to detect possible polymorphisms affecting sialic acid synthesis and to find a biomarker that may be related to Neu5Gc content in terms of animal breeding.A total of 11 single-nucleotide polymorphisms were found in sheep CMAH mRNA sequences. While 5 of the polymorphism positions cause amino acid changes, 6 of them are determined as synonymous single nucleotide polymorphisms. The polymorphism frequency results suggested that the AA genotype at position G27A in KD and MD (0.07 and 0.09, respectively), the GG genotype at position G55T in KD and MD (0.04 and 0.05, respectively), and the AA genotype at A294G in KD and MD (0.04 and 0.05, respectively) may be related to the amount of Neu5Gc sialic acid. In addition, alternative splicing variants have been demonstrated in the sheep CMAH gene, and it is thought that these variants also may affect the amount of Neu5Gc sialic acid.In conclusion, CMAH mRNA sequence was sequenced for the first time in Kıvırcık and Karacabey Merino sheep breeds and polymorphisms in exons were determined. Genotypes that may be related to the amount of Neu5Gc sialic acid were nominated for the first time in this study and it was mentioned for the first time that there are different mRNA variants of the sheep CMAH gene.

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