Potential Use of Nanofibrous Surfaces Containing Antibacterial Essential Oils as Wound Dressings

Project no. 32648 · Status: Kapanmış (Yayın bekleniyor) · 2024

Summary

<p>In&nbsp;this&nbsp;study,&nbsp;the&nbsp;potential&nbsp;application&nbsp;of&nbsp;antibacterial&nbsp;essential&nbsp;oil–loaded&nbsp;nanofibrous&nbsp;surfaces&nbsp;as&nbsp;wound&nbsp;dressings&nbsp;was&nbsp;investigated.&nbsp;Nanofibrous&nbsp;mats&nbsp;were&nbsp;fabricated&nbsp;via&nbsp;electrospinning&nbsp;using&nbsp;thermoplastic&nbsp;polyurethane&nbsp;(TPU)&nbsp;and&nbsp;polyvinyl&nbsp;alcohol&nbsp;(PVA)&nbsp;polymers,&nbsp;while&nbsp;tea&nbsp;tree&nbsp;oil,&nbsp;cinnamon&nbsp;oil,&nbsp;sage&nbsp;oil,&nbsp;and&nbsp;sage&nbsp;plant&nbsp;extract&nbsp;were&nbsp;incorporated&nbsp;as&nbsp;active&nbsp;agents.</p><p>The&nbsp;viscosity&nbsp;of&nbsp;the&nbsp;spinning&nbsp;solutions&nbsp;was&nbsp;evaluated,&nbsp;and&nbsp;the&nbsp;morphology&nbsp;of&nbsp;the&nbsp;produced&nbsp;nanofibrous&nbsp;surfaces&nbsp;was&nbsp;characterized&nbsp;by&nbsp;scanning&nbsp;electron&nbsp;microscopy&nbsp;(SEM).&nbsp;The&nbsp;chemical&nbsp;composition&nbsp;of&nbsp;the&nbsp;essential&nbsp;oils&nbsp;and&nbsp;nanofibrous&nbsp;structures&nbsp;was&nbsp;analyzed&nbsp;using&nbsp;gas&nbsp;chromatography–mass&nbsp;spectrometry&nbsp;(GC–MS)&nbsp;and&nbsp;Fourier&nbsp;transform&nbsp;infrared&nbsp;spectroscopy&nbsp;(FTIR),&nbsp;while&nbsp;their&nbsp;thermal&nbsp;properties&nbsp;were&nbsp;determined&nbsp;by&nbsp;thermogravimetric&nbsp;analysis&nbsp;(TGA).</p><p>Antibacterial&nbsp;activity&nbsp;was&nbsp;assessed&nbsp;by&nbsp;the&nbsp;agar&nbsp;diffusion&nbsp;method&nbsp;against&nbsp;<em>Staphylococcus&nbsp;aureus</em>&nbsp;(Gram-positive)&nbsp;and&nbsp;<em>Escherichia&nbsp;coli</em>&nbsp;(Gram-negative)&nbsp;bacteria.&nbsp;The&nbsp;results&nbsp;demonstrated&nbsp;that&nbsp;TPU&nbsp;nanofibrous&nbsp;surfaces&nbsp;loaded&nbsp;with&nbsp;tea&nbsp;tree&nbsp;oil&nbsp;and&nbsp;cinnamon&nbsp;oil&nbsp;exhibited&nbsp;significant&nbsp;antibacterial&nbsp;activity,&nbsp;whereas&nbsp;those&nbsp;containing&nbsp;sage&nbsp;oil&nbsp;and&nbsp;sage&nbsp;aqueous&nbsp;extract&nbsp;showed&nbsp;no&nbsp;antibacterial&nbsp;effect.&nbsp;Therefore,&nbsp;subsequent&nbsp;biological&nbsp;evaluations&nbsp;focused&nbsp;on&nbsp;nanofibrous&nbsp;surfaces&nbsp;incorporating&nbsp;tea&nbsp;tree&nbsp;oil&nbsp;and&nbsp;cinnamon&nbsp;oil.</p><p><em>In&nbsp;vitro</em>&nbsp;cytotoxicity,&nbsp;release&nbsp;profile,&nbsp;and&nbsp;wound&nbsp;healing&nbsp;assays,&nbsp;together&nbsp;with&nbsp;<em>in&nbsp;vivo</em>&nbsp;studies&nbsp;conducted&nbsp;on&nbsp;diabetic&nbsp;and&nbsp;non-diabetic&nbsp;animal&nbsp;models,&nbsp;revealed&nbsp;that&nbsp;the&nbsp;developed&nbsp;nanofibrous&nbsp;surfaces&nbsp;provided&nbsp;a&nbsp;supportive&nbsp;effect&nbsp;on&nbsp;wound&nbsp;healing.&nbsp;Notably,&nbsp;tea&nbsp;tree&nbsp;oil–loaded&nbsp;nanofibrous&nbsp;surfaces&nbsp;demonstrated&nbsp;a&nbsp;more&nbsp;pronounced&nbsp;wound&nbsp;healing&nbsp;performance&nbsp;compared&nbsp;to&nbsp;cinnamon&nbsp;oil–loaded&nbsp;counterparts.&nbsp;Overall,&nbsp;these&nbsp;findings&nbsp;indicate&nbsp;that&nbsp;essential&nbsp;oil–loaded&nbsp;TPU&nbsp;nanofibrous&nbsp;surfaces&nbsp;are&nbsp;functionally&nbsp;and&nbsp;biologically&nbsp;effective,&nbsp;highlighting&nbsp;their&nbsp;strong&nbsp;potential&nbsp;as&nbsp;advanced&nbsp;wound&nbsp;dressing&nbsp;materials.</p>

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