ISOLATION, SCREENING AND OPTIMIZATION OF PRODUCTION MEDIA FOR BIOACTIVE COMPOUNDS FROM ACTINOMYCETES ISOLATED FROM AQUATIC ENVIRONMENTS
Project no. 437 · Status: Kapanmış · 2017
Summary
In this thesis, the isolation of microorganisms which are the member of actinomycetes, the screening of antimicrobial activities and optimization of production medium has been carried out. 16 actinomycete isolates were obtained from lagoon sediment which is located in Inciralti, Izmir region. Primary antimicrobial activity was screened using the Cross-streak method. According to the primary antimicrobial activity screening results, the ERS13 isolate has showed the highest activity. According to phenotypic and genotypic characteristics of the isolate; it has been determined that it is whitish in colour, aerobic, pellet forming in broth culture, non motile, filamentous, gram positive and 99% similar to Streptomyces variabilis strain VITUMVB03. Disc diffusion method was used for secondary antimicrobial activity screening. The ERS13 isolate has showed the highest inhibition zone against Bacillus cereus ATCC 7064, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 29998, Salmonella typhimurium, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 6538/P, Aspergillus niger and Candida albicans with a zone of inhibition of 10 mm, 24 mm, 13 mm, 15 mm, 20 mm, 16 mm, 18 mm ve 12 mm, respectively. The production medium optimization was determined by disc diffusion test using extracts of the ERS13 isolate. According to the test results, Carbohydrate carcode medium (CCM) was determined as the most active production medium which contain soyaflour (20.0 g/l), glucose (5.0 g/l), ribose (5.0 g/l), glycerol (5.0 g/l), potato starch (5.0 g/l), ammonium sulphate (0.2 g/l), yeast extract (2.0 g/l) and Bactopeptone (2.0 g/l). Keywords: Bioactive compound, Extraction, Secondary metabolite, Disc diffusion, Aquatic actinomycete, Cross-streak method.