As a Multifunctional Theranostic Agent; Nanoencapsulation of Lycopene in Niosomes and Determination of Antitumor Activity on Cancer Cells
Project no. 20838 · Status: Kapanmış · 2019
Summary
Within the scope of the thesis, it was aimed to determine anti-proliferative and apoptotic effects of phytochemical agent lycopene on the prostate cell lines. Vesicular nano carriers called niosomes were used to maintain stability of lycopene to target to prostate cells and obtain fluorescence images. Monoclonal anti-PSMA antibody conjugation onto niosomes was performed to provide specificity to PSMA + cells, and indocyanine green encapsulation (ICG) was performed for fluorescence imaging. Cellular incorporation levels of the vesicular system were determined spectrophotometrically. Niosome characterization procedures were performed by DLS, SEM, FTIR, XPS, drug encapsulation efficiency and drug release profile study. MTT study was conducted to determine the antiproliferative effect of the characterized niosomes. AnnexinV/7-AAD apoptosis kit was used to determine the apoptotic effect of niosomes. Cellular uptake/association of niosomes was done by fluorescent imaging and fluorescent incorporation study.