Determination of the biodistribution of Alpha-amanitine in mice
Project no. 23987 · Status: Kapanmış · 2022
Summary
<p>Mushroom poisonings caused by Amanita phalloides are the leading cause of mushroom-related deaths worldwide. Alpha-Amanitin (α-AMA), a toxic substance present in these mushrooms, is responsible for the resulting hepatotoxicity and nephrotoxicity. The aim of this study was to radiolabel α-AMA with Iodine-131 and to determine the biodistribution of radiolabeled α-AMA in Balb/c mice. For this purpose, α-AMA was labeled with I-131 radionuclide with a radiolabeling efficiency of 94.5±4.0% according to the iodogen method. In biodistribution studies of the radiolabeled toxin, mice were injected with a toxic dose (1.4 mg/kg) of Iodine-131 labeled α-AMA. The mice were sacrificed at 1st, 2nd, 4th, 8th, 24th and 48th hours under anesthesia, their organs were removed and injected dose/gram (%ID/g) values were calculated. The %ID/g values for kidney, liver, lung, and heart tissues at 1 hour were 1.59±0.07, 1.25±0.33, 3.67±0.80, and 1.07±0.01, respectively. In addition, this study provides insight into the potential long-term effects of α-AMA accumulation in specific organs. Thus, it can help patient management and improve clinical outcomes. In addition, this study highlights the importance of understanding the biodistribution of toxins at toxic doses to develop targeted therapies and improve patient outcomes.</p>