CALLUS PRODUCTION OF Corallina officinalis L. AND DETERMINATION OF ANTIOXIDANT, ANTIMICRIBIAL BIOACTIVITY
Project no. 22197 · Status: Kapanmış (Yayın bekleniyor) · 2020
Summary
Corallina officinalis is a calcareous, red algae of the phylum Rhodophyta, up to 12 centimeters tall, but generally shorter. Individuals of the species grow around tide pools or are found in shallow crevices of coastal cliffs that are regularly replenished by seawater. Optimal culture conditions and regeneration properties of algal explants were studied in order to establish the micropropagation system of Corallina officinalis with tissue culture techniques and to perform axial (single type cell culture) culture of this species. PES containing germanium dioxide (Provasoli, 1968) and MCM (Marine Culture Medium) (McLahlan, 1973) medium were tried to evaluate the best callus medium from Corallina officinalis, a marine algae whose callus culture was not obtained before, and the best callus medium reported as PES P1 with 16% calli yield (1:0,1 g/l) semi-liquid medium. In order to examine the regeneration process, polarity and optimal lighting levels, regenerative structures were observed under the microscope and counted at regular intervals and optimized conditions were determined. Production of callus from thallus was accomplished by cutting the thallus into 2 or 3 mm pieces and transferring them to sterile test tubes with growth regulator supplements as auxin: cytokinin (1:0,1; 1,5:0,5; 1:2) for the 0,04% agar substrate. Callus was formed from the plant thallus in callus the media of M2 (1,5:0,5 g/l), M3 (2:1 g/l), P1(1:0,1 g/l) Corallina officinalis. All subcultures of callus initiation and fragile callus cells from liquid medium were provided in vitro and standardized. At the end of the 2-month growth period following callus formation, the cultures were subcultured and fresh and dry weight data were obtained. The growth rate from the apical and node was determined. Antioxidant activities were determined as 32% in 160 mg; 16% in 80 mg and antimicrobial activities were as MIC value 3 mg/ml for S. aureus, 6mg/ml for E. coli and 6 mg/ml for E. faecalis and the highest MIC value was 12 mg/ml for C. albicans