Avrupa Levreği'nde (Dicentrarchus labrax) Büyüme Hormonu (GH) ve Miyostatin (MSTN) Genlerindeki Polimorfizimler ile Büyüme Özellikleri Arasındaki İlişkilerin Belirlenmesi
Project no. 21912 · Status: Kapanmış · 2020
Summary
In this project, single nucleotide polymorphisms (SNPs) in European Sea Bass (Dicentrarchus labrax) Growth Hormone (GH) gene and Myostatin (MSTN) genes were determined by DNA sequencing method and possible associations between the growth characteristics and these SNPs were investigated. In the study, a total of 10 SNPs were determined in the of 576 bp of GH gene region, which includes the 1st intron, 2nd exon and intron, partial 3rd exon of 200 European sea bass. GH gene 1st intron; g.1557 A>T loci was associated with abdominal length and pre-anal length, g.1611 T>C loci was associated with total weight, fillet weight, head length; g.1663 C>G loci was associated with abdominal length. The GH gene g.1663 C>G loci and the g.1799 T>C nucleotide change which causes a nonsynonymous amino acid change from Leucine (L) to Serine (S) in the second exon and body length was found associated (p<0.05). According to the results of the sequence analysis of 2375 bp region of the MSTN gene, which includes the 1st, 2nd and 3rd exon regions of this gene; a total of 9 SNPs and 2 indels were detected. The associations between the standard length and g.16612A indel located in the 1st intron region; the MSTN g.15252T>A locus in the 2nd intron region and the total weight, fillet weight and standard length was determined (p<0.05). The relationship of the g.14873C>T loci in the 3rd exon of the GH gene and standard height, head length, body length, pre-anal length, abdominal length, post-anal length, and head width was found to be significant (p<0.05). GH and MSTN genes are important candidate genes for growth traits in aquaculture species. Therefore, it is suggested that SNPs identified in both gene regions in the study should be evaluated as candidate SNPs for European sea bass breeding and marker assisted selection (MAS).