EVALUATION OF DIFFERENT VEGETABLE OIL MEALS IN TERMS OF BIOACTIVE PEPTIDE POTENTIAL
Project no. 703 · Status: Kapanmış · 2015
Summary
In this project, bioactive peptide formation potentials of sunflower and hazelnut meals, which are classified as by-products of vegetable oil industry and which are considered as animal feed due to their high protein content, have been investigated. The protein content of the isolates obtained from sunflower and hazelnut meal by alkali extraction and isoelectric precipitation were 96% and 94.2%, respectively. The hydrolysis process applied to the isolates with Alcalase, Protamex and Trypsin+Chymotrypsin enzymes revealed that the highest degree of hydrolysis for sunflower and hazelnut proteins was 27% and 19%, respectively. The <5 kDa fractions of Alcalase and Trypsin+Chymotrypsin treated protein hydrolysates showed ACE inhibitory (IC50: 63-176 µg/ mL), DPP-IV inhibitory (IC50: 0.28-0.52 mg / mL) and total antioxidant (ORAC: 2-3.52 µmol Trolox/mg) activities. For both samples, inhibition of ACE was higher for hydrolysates obtained with Trypsin+Chymotrypsin, whereas DPP-IV inhibition was higher in Alcalase hydrolysates. The results of the project have shown that both proteins are a potential source for bioactive peptide delivery and that the peptides obtained can be used as an alternative source to the development of new functional products.