Cholinergic Signaling Mechanisms and Early Implant Osseointegration in Healthy Versus Generalized Aggressive Periodontitis Patients: A prospective, case-control study
Project no. 231 · Status: Kapanmış · 2017
Summary
Aims Periodontal diseases negatively affect implant osseointegration. Perturbations in non-neuronal cholinergic signaling mechanisms are associated with periodontitis; however, their role in generalized aggressive periodontitis (GAgP) is unknown. The aim of this prospective and case-control study was to determine the relationship between non-neuronal cholinergic signaling mechanisms, interleukin-17A (IL-17A) levels and early implant osseointegration in GAgP. Materials and Methods Thirteen GAgP patients and seven periodontally healthy individuals (PH) were recruited. Peri-implant crevicular fluid (PICF) was obtained at baseline and 1-month post-placement. Acetylcholine (ACh) levels and cholinesterase activity were determined by biochemical assays. Secreted Ly-6/uPAR-related protein-1 (SLURP-1), IL-17A and IL-17E levels were determined by ELISA. Marginal bone loss (MBL) at 1-month and 6-months post-placement was determined radiographically. Results The concentration of ACh, cholinesterase activity levels and IL-17A levels were elevated in PICF of patients with GAgP at baseline and 1-month post-placement compare to PH individuals. The concentration of ACh and cholinesterase activity levels in PICF correlated with levels of IL-17A and marginal bone loss around implants 1-month post-placement in patients with GAgP. Conclusions Non-neuronal cholinergic mechanisms may play a role in the aetiopathogenesis of GAgP and may directly or indirectly, through modulation of IL-17A, influence early implant osseointegration and potential long-term implant survival.