INVESTIGATING THE EFFECTS OF NICOTINE INTAKE THROUGH GENERATIONS TO ALCOHOL CONSUMPTION
Project no. 126 · Status: Kapanmış · 2017
Summary
Alcohol and cigarettes are the leading substances that are abused together. Twin studies suggest that shared genetic vulnerability underlies alcohol and nicotine addiction. Previously, Shaham et al. showed that alcohol-naive offspring of rats genetically selected for high alcohol intake are susceptible to nicotine self-administration. However there is no study for offspring of high nicotine preferring line(P). Here, we aim to find the difference of oral alcohol intake between nicotine-naive offspring of P and naive male/female rats in adulthood. P rats have been selectively bred for high oral nicotine intake in Ege University. Nine female and 8 male P Sprague Dawley rats from 18th generation and 20 naive SD male/female rats were used. Twelve weeks old rats were given intermittent access to 20% ethanol in a 2-bottle choice procedure (IA2BC). Fluid intake measured 1 hour and 24 hour after starting session and converted into mlkg. Weight changes measured on a weekly basis. Statistical analysis was performed using repeated measures ANOVA on SPSS package program. Results show that for 24 hour sessions P rats drink more alcohol than control rats(f (1,33)=59,554 p?0,001) and control rats drink more water than P rats(f (1,33)=83,758p?0,001). Female rats drink more alcohol than male rats (f (1,33)=11,719 p?0,005) and female control rats drink more water than male control and P male/female rats (p?0.05). Results for 1 hour sessions are similar. Our study supports the hypothesis that shared genetic determinant accounts for the co-abuse of nicotine and alcohol and provides experimental data for further genetic studies.