HN1 INFLUENCES CELL CYCLE REGULATION IN SH-SY5Y (NEUROBLASTOMA) CELL LINE
Project no. 23341 · Status: Kapanmış (Yayın bekleniyor) · 2022
Summary
<p>The cell cycle is the system controlled by the mechanisms in which many molecules take part in order for cells to divide correctly. Disorders in the mechanisms which are controlled cell division might leads to the formation of abnormal cells. According to this, this kind of formations leads to many serious diseases such as cancer. In the cell cycle, the functioning of all these mechanisms is controlled by many different regulatory proteins. Degradation of cell cycle regulatory proteins levels are highly variable due to the phase-dependent expression. Selective and programmed protein degradation in different phases of the cell cycle is accomplished by directing it to the proteosome under the E3 ubiquitin ligase complex. Activation of the Parkin complex which is the E3 ubiquitin protein ligase is necessary for the continuation of the cell cycle. In case of parkin deficiency, mitotic protein levels are high, followed by multiple mitotic defects. Hematologically and neurologically expressed sequence 1 (HN1) is a protein that has important role in cell cycle and centrosome development. In this thesis study, HN1 co-expression network obtained from neuroblastoma patients by in silico approaches has been shown to be involved in many cell cycle-related biological processes. The inducible Tet-ON lentivirus vector system has been established to ensure high expression of HN1 in SH-SY5Y cells. In SH-SY5Y cells, the phenotype that develops in case of high expression of HN1 was characterized and the effect of HN1 level on Parkin as the cell cycle progressed. In conclusion, level of the HN1 in SH-SY5Y cells is significant for the controlled progress of the cell cycle. In cell cycle, HN1 and Parkin phase-dependent expression profile. The effect of HN1 on Parkin is significant due to the increase in the level of Cdh1 which is Parkins coactivator with HN1 overexpression.</p>