In silico Identification and in vivo validation of Fucosyltransferase in Some Insect Species

Project no. 27378 · Status: Kapanmış · 2023

Summary

Fucosyltransferases (FucTs) are type II trans-membrane Golgi-resident proteins that catalyze the transfer (fucosylation) of α-L-fucose from GDP-Fuc onto N- and O-linked glycans, free oligosaccharides, or lipids or directly onto proteins. Fucosylations are involved in a variety of biological processes, including selectin-mediated leukocyte-endothelial adhesion, lymphocyte homing, ABO blood group histocompatibility, notch receptor signaling, embryo-fetal development, and host-microbe interactions.The FUT gene family encodes fucosyltransferase proteins. In mammals, 13 fucosyltransferase gene families (FUT) have been identified, including FUT 1-11, POFUT1 (Protein O-fucosyltransferase 1) and POFUT2 (Protein O-fucosyltransferase 2). In insects, there are few studies on fucosyltransferase and FUT types are not characterized by species. The purpose of the article is to determine which FUTs found in insects have a common gene structure with the FUT gene family found in mammals, and to identify and characterize levels in Bombyx mori larvae were determined by QPCR.Fucosyltransferase motif sequences were determined from the transcriptomes (all mRNA sequences) obtained from the databases of insects with known genomes through databases (Clustal Omega, Prosite and MEME-Suite/FIMO), and possible fucosyltransferases in insects were determined. Fucosyltransferases in insects were compared with 13 fucosyltransferase sequences in mammals. Then, the similarities between the three-dimensional protein structures of the mammalian fucosyltransferase enzymes (Swiss-Model, AlphaFold and pyMOL databases) were revealed.Similarities to those in mammals were obtained in motifs found in fucosyltransferases in insects. According to the patterns and three-dimensional structure comparison regions, all 13 fucosyltransferases were found in Callosobruchus maculatus, FucT1 and FucT9 in Galleria mellonella, FucT1, FucT4, FucT5, FucT8 and FucT11, FucT2, FucT6, FucT in Manduca sexta, in Myzus persicae. FucT9, FucT10 and FucT11, FucT1, FucT3, FucT5, FucT6 and FucT9 motifs have been characterized in Bombyx mori species. Considering the real-time PCR results, α1,6 and α1,3-fucosyltransferase gene expression levels differ in different tissues of silkworm (B. mori) on different days.This information provides guidance for continuing studies on fucosyltransferases in invertebrates. New sequences for the fucosyltransferase enzyme will be revealed as the genome of determined insects is studied. While the similarities of fucosyltransferase motifs in insects and mammals indicate that they may be orthologous genes in terms of evolution, it is also an indication that new developmental and evolutionary studies will be conducted on this subject.

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