INVESTIGATION OF EFFECTS OF KOLLIPHOR ON CELL VIABILITY AND CASPASE-3 ENZYME IN PC-12 CELLS
Project no. 493 · Status: Kapanmış · 2018
Summary
Kolliphor is a polyethoxylated form of oil used in food and cosmetics, also known as castor oil. Kolliphor is used as a solvent and carrier agent for many water-insoluble oil-soluble drugs. It is also known that kolliphor is toxic without combination of drugs. PC12 cells are a valuable model to mimic nerve cell toxicity with the ability to create neuronal-like cells. PC-12 cells were grown in complete medium containing RPMI-1640 and 10% HS, 5% FBS, 1% antibiotic. Kollifor was administered in varying doses (0.01%; 0.02%; 0.05% v / v) in solution. For this purpose, cells were cultured in 96-well culture plates for 24 and 48 hours. Cells were seeded at 10000 cells per well. The control group received the same volume of sterile water. The ELISA plate reader was used for WST-1 test at 450 nm. Total mRNA was isolated from the passaged cells under the same conditions and expression of ß-Actin and caspase-3 genes by qRT-PCR was examined. According to the results of cell viability, increased toxicity was observed with the doses administered and applied. Additionally, caspase-3 expression was increased with 0.05% v/v of kolliphor application after 48 hours incubation. According to these results, it is possible to suggest that increasing doses of kolliphor administration are toxic for PC12 cells and route the cells to apoptosis after 48 hours.