Development of a Controlled Drug Delivery System for the Treatment of Periodontitis

Project no. 23471 · Status: Kapanmış (Yayın bekleniyor) · 2022

Summary

<p>Within&nbsp;the&nbsp;scope&nbsp;of&nbsp;this&nbsp;project,&nbsp;a&nbsp;system&nbsp;providing&nbsp;controlled&nbsp;and&nbsp;combined&nbsp;drug&nbsp;release&nbsp;for&nbsp;the&nbsp;treatment&nbsp;of&nbsp;periodontal&nbsp;diseases&nbsp;was&nbsp;developed.&nbsp;Poly(lactic&nbsp;acid)&nbsp;(PLA)-based&nbsp;nanogels&nbsp;functionalized&nbsp;with&nbsp;acrylate&nbsp;and&nbsp;glucosamine&nbsp;and&nbsp;loaded&nbsp;with&nbsp;doxycycline&nbsp;and&nbsp;simvastatin,&nbsp;possessing&nbsp;antibiotic&nbsp;and&nbsp;anti-inflammatory&nbsp;properties,&nbsp;were&nbsp;successfully&nbsp;prepared.&nbsp;PLA&nbsp;polymers&nbsp;functionalized&nbsp;with&nbsp;glucosamine&nbsp;to&nbsp;provide&nbsp;bioadhesive&nbsp;properties&nbsp;and&nbsp;with&nbsp;acrylate&nbsp;groups&nbsp;to&nbsp;confer&nbsp;antimicrobial&nbsp;activity&nbsp;were&nbsp;synthesized&nbsp;and&nbsp;combined&nbsp;at&nbsp;a&nbsp;50%&nbsp;ratio&nbsp;to&nbsp;obtain&nbsp;nanogel&nbsp;formulations&nbsp;using&nbsp;the&nbsp;emulsion–solvent&nbsp;evaporation&nbsp;method.&nbsp;The&nbsp;prepared&nbsp;nanogels&nbsp;were&nbsp;evaluated&nbsp;under&nbsp;in&nbsp;vitro&nbsp;conditions&nbsp;in&nbsp;artificial&nbsp;saliva&nbsp;for&nbsp;their&nbsp;72-hour&nbsp;release&nbsp;kinetics,&nbsp;stability,&nbsp;sterilization&nbsp;compatibility,&nbsp;and&nbsp;bioadhesion&nbsp;properties.&nbsp;Their&nbsp;antimicrobial&nbsp;activities&nbsp;were&nbsp;investigated&nbsp;against&nbsp;P.&nbsp;gingivalis,&nbsp;S.&nbsp;aureus,&nbsp;E.&nbsp;coli,&nbsp;P.&nbsp;aeruginosa,&nbsp;E.&nbsp;faecalis,&nbsp;and&nbsp;A.&nbsp;actinomycetemcomitans,&nbsp;and&nbsp;their&nbsp;minimum&nbsp;biofilm&nbsp;inhibitory&nbsp;concentrations&nbsp;and&nbsp;effects&nbsp;on&nbsp;biofilm&nbsp;formation&nbsp;were&nbsp;determined.&nbsp;Based&nbsp;on&nbsp;the&nbsp;project&nbsp;outcomes,&nbsp;in&nbsp;vivo&nbsp;testing&nbsp;of&nbsp;the&nbsp;developed&nbsp;product&nbsp;at&nbsp;the&nbsp;Forsyth&nbsp;Institute&nbsp;(USA)&nbsp;was&nbsp;planned,&nbsp;and&nbsp;periodontitis&nbsp;animal&nbsp;models&nbsp;were&nbsp;established&nbsp;for&nbsp;this&nbsp;purpose.</p>

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