RESEARCH OF THE EFFECTS OF APELIN ON ISCHEMIA/REPERFUSION INJURY
Project no. 22740 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
In Plastic and Reconstructive Surgery practice, flap surgery is performed very frequently after tissue loss in cases such as trauma, tumor resection, etc. Over time, the tools used with the advancement of technology were also developed and over time, there were groundbreaking changes in flap surgery. In our experimental model, 28 Wistar albino type rats were used to obtain biostatistically significant results. 4 separate groups were created. In the first group, the inguinal flap was elevated without any ischemic insult. The flap was then elevated till femoral artery and vein so that it would be a flow-through flap without any ischemia. In rats in the second group, the inguinal flap was elevated the same way. Vascular anastomoses were performed after a 2 hours of ischemia period. In rats of the third group, the free flap was elevated from the inguinal region, ischemia was applied for 2-hours, and Apelin-free gel formulation was applied to the subcutanous plane after vascular anastomosis. In the fourth group, the same procedures were done together with the gel formulation containing apelin. After seven days, all the flaps were checked, and excised, and after macroscopic examinations, flaps were put into formaldehyde solution and routine histological follow-up. In histological evaluation, samples stained with hemotoxylene-eosin and Mallory azan were evaluated histopathologically with modified Verhofstad Scoring. In addition, sections were stained with anti e-NOS and anti-VEGF immunohistochemical dyes. When the averages of the scores were calculated by performing modified verhostad scoring on the samples, it was seen that the 4th group received fewer ischemia-reperfusion scores than the 2nd group. With these findings, it was concluded that the use of apelin gel formulation had a meaningful effect in order to prevent the development of ischemia-reperfusion damage.