Investigation of The Effects on Prostate Cancer Development of MicroRNA Profiles Varying in The Inflammatory Micro Environment
Project no. 22637 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
<p>Project Scope: Our project covers the relationship between microRNAs (miRNA) and inflammation-induced epithelial-mesenchymal transition (EMT) in the tumor microenvironment, which is one of the causes of prostate cancer (PCa) metastasis, and the determination of the therapeutic potential of miRNAs in the treatment of PCa.</p><p><br></p><p>Method: In the preliminary study of the project, 16 mRNAs and 6 miRNAs responsible for androgen regulation in LNCaP cells were determined by bioinformatics analysis from microarray data and GSE16225 data set. After transfection of the determined miRNAs into cells, gene and protein changes in EMT signaling pathways were examined by qRT-PCR and Western Blot analyses. EGF, hypoxia, and synthetic androgen (R1881) were administered to induce metastasis. In addition, changes in cell metastasis were investigated by examining cell proliferation, cell migration, and invasion to understand the morphological effects on the cells.</p><p><br></p><p>Original Value: External factors such as hypoxic environmental conditions and disruption of hormone balance cause inflammation-induced EMT and metastasis. To date, the relationship of these external factors with MiR-27b and MiR-133b has not been revealed. For this reason, our study has unique values and provides findings that will help develop more effective therapeutic strategies for prostate cancer. The effect of the identified miRNAs on signaling pathways in the inflammatory microenvironment and their potential for use in prostate cancer metastasis were revealed.</p><p><br></p><p>Results: With miRNA transfection in our project work packages, mesenchymal properties were lost in PCa cells and epithelial properties were restored to the cells. In addition, cell migration, which forms the basis of metastasis, is prevented. MiR-27b and MiR-133b have slowed cancer cell proliferation, migration, invasion, and wound healing. It is thought that increasing the use of these miRNAs will contribute to developing treatment methods for PCa patients, which is also common in our country.</p>