Synthesis of Zinc Oxide Nanoparticles from Lactic Acid Bacteria Isolated from Various Foods and Investigation of Their in-vitro Efficacy Against the Acne Pathogen Cutibacterium acnes

Project no. 32720 · Status: Kapanmış (Yayın bekleniyor) · 2024

Summary

<p><span style="color: rgb(0, 0, 0);">Acne&nbsp;vulgaris&nbsp;is&nbsp;a&nbsp;chronic&nbsp;inflammatory&nbsp;skin&nbsp;disorder&nbsp;in&nbsp;which&nbsp;</span><em style="color: rgb(0, 0, 0);">Cutibacterium&nbsp;acnes</em><span style="color: rgb(0, 0, 0);">&nbsp;plays&nbsp;a&nbsp;major&nbsp;role&nbsp;in&nbsp;disease&nbsp;pathogenesis.&nbsp;It&nbsp;ranks&nbsp;among&nbsp;the&nbsp;most&nbsp;prevalent&nbsp;diseases&nbsp;worldwide&nbsp;and&nbsp;can&nbsp;negatively&nbsp;a!ect&nbsp;individuals&nbsp;not&nbsp;only&nbsp;physically&nbsp;but&nbsp;also&nbsp;psychologically&nbsp;by&nbsp;reducing&nbsp;their&nbsp;quality&nbsp;of&nbsp;life&nbsp;and&nbsp;emotional&nbsp;well-being.&nbsp;The&nbsp;prolonged&nbsp;and&nbsp;inappropriate&nbsp;use&nbsp;of&nbsp;antibiotics&nbsp;in&nbsp;acne&nbsp;treatment&nbsp;may&nbsp;promote&nbsp;the&nbsp;emergence&nbsp;of&nbsp;antibiotic-resistant&nbsp;microorganisms.&nbsp;Increasing&nbsp;antimicrobial&nbsp;resistance&nbsp;can&nbsp;limit&nbsp;the&nbsp;therapeutic&nbsp;efficacy&nbsp;of&nbsp;conventional&nbsp;treatments&nbsp;and&nbsp;highlights&nbsp;the&nbsp;need&nbsp;to&nbsp;develop&nbsp;alternative&nbsp;antimicrobial&nbsp;strategies.&nbsp;In&nbsp;this&nbsp;context,&nbsp;nanoparticles&nbsp;produced&nbsp;through&nbsp;green&nbsp;synthesis&nbsp;have&nbsp;attracted&nbsp;considerable&nbsp;attention&nbsp;as&nbsp;environmentally&nbsp;sustainable&nbsp;antibacterial&nbsp;agents&nbsp;because&nbsp;these&nbsp;approaches&nbsp;can&nbsp;reduce&nbsp;the&nbsp;toxic&nbsp;by-products&nbsp;and&nbsp;hazardous&nbsp;waste&nbsp;associated&nbsp;with&nbsp;conventional&nbsp;chemical&nbsp;synthesis&nbsp;methods.</span></p><p></p><p><span style="color: rgb(0, 0, 0);">In&nbsp;the&nbsp;present&nbsp;study,&nbsp;zinc&nbsp;oxide&nbsp;nanoparticles&nbsp;(ZnO&nbsp;NPs)&nbsp;were&nbsp;biologically&nbsp;synthesized&nbsp;using&nbsp;extracellular&nbsp;bioactive&nbsp;compounds&nbsp;produced&nbsp;by&nbsp;lactic&nbsp;acid&nbsp;bacteria.&nbsp;UV–visible&nbsp;spectrophotometry&nbsp;and&nbsp;scanning&nbsp;electron&nbsp;microscopy&nbsp;(SEM)&nbsp;were&nbsp;employed&nbsp;to&nbsp;confirm&nbsp;nanoparticle&nbsp;formation&nbsp;and&nbsp;characterize&nbsp;the&nbsp;resulting&nbsp;structures,&nbsp;while&nbsp;Fourier-transform&nbsp;infrared&nbsp;spectroscopy&nbsp;(FT-IR)&nbsp;was&nbsp;used&nbsp;to&nbsp;identify&nbsp;the&nbsp;functional&nbsp;groups&nbsp;associated&nbsp;with&nbsp;the&nbsp;synthesized&nbsp;nanoparticles.&nbsp;The&nbsp;antibacterial&nbsp;activity&nbsp;of&nbsp;ZnO&nbsp;NPs&nbsp;synthesized&nbsp;using&nbsp;isolates&nbsp;obtained&nbsp;from&nbsp;Tulum&nbsp;cheese&nbsp;and&nbsp;identified&nbsp;by&nbsp;sequence&nbsp;analysis&nbsp;as&nbsp;</span><em style="color: rgb(0, 0, 0);">Lentilactobacillus&nbsp;kefiri</em><span style="color: rgb(0, 0, 0);">&nbsp;and&nbsp;</span><em style="color: rgb(0, 0, 0);">Lactococcus&nbsp;lactis</em><span style="color: rgb(0, 0, 0);">&nbsp;was&nbsp;evaluated&nbsp;against&nbsp;</span><em style="color: rgb(0, 0, 0);">C.&nbsp;acnes&nbsp;</em><span style="color: rgb(0, 0, 0);">ATCC&nbsp;11827.&nbsp;The&nbsp;ZnO&nbsp;NPs&nbsp;synthesized&nbsp;using&nbsp;</span><em style="color: rgb(0, 0, 0);">Lentilactobacillus&nbsp;kefiri&nbsp;</em><span style="color: rgb(0, 0, 0);">and&nbsp;</span><em style="color: rgb(0, 0, 0);">Lactococcus&nbsp;lactis&nbsp;</em><span style="color: rgb(0, 0, 0);">produced&nbsp;inhibition&nbsp;zones&nbsp;of&nbsp;13.0&nbsp;±&nbsp;0.17&nbsp;mm&nbsp;and&nbsp;9.0&nbsp;±&nbsp;0.15&nbsp;mm,&nbsp;respectively.&nbsp;Their&nbsp;minimum&nbsp;inhibitory&nbsp;concentrations&nbsp;were&nbsp;determined&nbsp;as&nbsp;0.125&nbsp;mg/mL&nbsp;and&nbsp;0.25&nbsp;mg/mL,&nbsp;respectively.&nbsp;Furthermore,&nbsp;at&nbsp;concentrations&nbsp;corresponding&nbsp;to&nbsp;10&nbsp;×&nbsp;MIC,&nbsp;both&nbsp;ZnO&nbsp;NP&nbsp;preparations&nbsp;caused&nbsp;a&nbsp;&gt;4.49&nbsp;log&nbsp;reduction&nbsp;in&nbsp;the&nbsp;viable&nbsp;bacterial&nbsp;population&nbsp;within&nbsp;3&nbsp;h,&nbsp;reducing&nbsp;the&nbsp;bacterial&nbsp;counts&nbsp;to&nbsp;below&nbsp;the&nbsp;detection&nbsp;limit.</span></p><p></p><p></p>

View details