The Importance Of Certain miRNAs in circulation on the Development and Dıagnosıs Of Pulmonary Arterial Hypertension
Project no. 38 · Status: Kapanmış · 2016
Summary
Pulmonery arteriyel hypertension (PAH) is characterized by maladaptation of pulmonery vascularute which is leading to right ventricular hypertrophy and hearth failure. MicroRNAs (miRNA) are the small non-coding RNAs which consist of ~22 nucleotides, regulate the gene expression negatively at the transcriptional or post-transcriptional level. miRNAs play a crucial role in the regulation of many diseases such as viral infection, cancer, cardiovascular diseases,and PAH. In this study, we aimed to investigate the expression pattern of eight human plasma (hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p) miRNAs in mild-to-severe PAH patients and healty controls. miRNAs were extracted from peripheral plasma of the PAH patients (n:50) and healty individuals (n:30) by using the miRNA Isolation Kit. cDNA was synthesised using Allin-One First strand cDNA Synthesis Kit. Expression of the human plasma hsa-miR-21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p miRNAs were analysed by qRT-PCR. According to our results, in PAH patients hsa-miR-21-3p and hsa-miR-143-3p expressions levels were decreased 4 and 2 times, respectively. No significant changes were detected in hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsa-miR-206, hsa-miR-210-3p expression levels between patient and control group. In the early diagnosis of PAH, hsa-miR-21-3p and hsa-miR-143-3p in peripheral plasma can be considered as potential biomarkers.