IN VITRO CLONAL MICROPROPAGATION OF DIFFERENT IZMIR OREGANO (ORIGANUM ONITES L.) CULTIVARS
Project no. 22541 · Status: Kapanmış · 2020
Summary
<p>This thesis study aims to establish a successful protocol for in vitro clonal </p><p>micropropagation procedure in elite cultivars of Origanum onites L. (İzmir oregano). </p><p><br></p><p>Node explants of 2 cultivars (Ceylan 2002 and Tayşi 2002) belonging to Origanum onites </p><p>L. species are subjected to different sterilization methods. A sterilization success of 100% in </p><p>Ceylan 2002 cultivar and 90% in Tayşi 2002 cultivar is achieved. In Ceylan 2002 cultivar, the </p><p>highest shoot regeneration percentage (13,33%), the highest average shoot length (0,12 cm) and </p><p>the highest average leaf number (0,87), is achieved in MS nutrient medium containing 0,3 g/L </p><p>activated carbon supplemented with 2,0 mg/L BAP. No shoot regeneration is achieved in Tayşi </p><p>2002 cultivar. In Ceylan 2002 cultivar, the highest propagation coefficient (3,40) in the 4th </p><p>subculture, the highest average shoot length (3,82 cm) and number of leaves per explant (13,85) </p><p>in the 3rd subculture, the highest average root number (0,81) and root length (1,99 cm) per </p><p>explant in the 6th subculture, the highest number of shoots per node (0,47) in the 8th subculture </p><p>is reached. The highest percentage of root regeneration is observed as 100%. The highest </p><p>average root number per explant (12,03) value is obtained in MS nutrient medium containing </p><p>1,0 g/L activated carbon supplemented with 2,0 mg/L IBA. The highest average root length </p><p>(2,72 cm) is achieved in MS medium containing 1,0 g/L activated carbon supplemented with </p><p>0,5 mg/L IBA.The highest average stem thickness value (2,02 mm) and the highest average </p><p>shoot length (10,02 cm) are obtained in nutrient media containing 1,0 g/L activated carbon.The </p><p>rooted plantlets are acclimatized with 16,25% success percentage. </p><p><br></p><p>The highest essential oil content (2.00%) is observed in regenerated shoots obtained from </p><p>MS nutrient media supplemented with 2,0 mg/L IBA. The highest thymol ratio (65.33%) is </p><p>found in regenerated shoots obtained from ½ MS nutrient media supplemented with 0,5 mg/L </p><p>IBA; the highest carvacrol ratio (6.82%) is achieved in regenerated shoots obtained from MS </p><p>nutrient media supplemented with 2,0 mg/L IBA. </p>