LEVETIRASETAM VE LAMOTRİJİNİN SIÇANLARDA İLK TRİMESTERDEKİ NÖRAL TÜP GELİŞİMİ VE NÖRAL KREST HÜCRE GÖÇÜ ÜZERİNE ETKİLERİNİN İNCELENMESİ
Project no. 23103 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
<p>In this study, it was aimed to examine the effects of low and high doses of anti-epileptic drugs with different mechanisms on neurulation and during neural crest cell migration. The long-term general aim is to obtain low and high-dose embryological data of common agents that are not in the literature and high-dose of agents considered safe in the literature in order to contribute to the social and medical well-being of families, where human health is at the forefront. As a method, pre-pregnancy follow-up was done with cytopathology, in which the whole cycle was carefully examined, and pregnancy follow-up was made with</p><p>ultrasonographic methods. Maternal weights were checked regularly. Routine histological methods were used to generate histological data and tissues were embedded in paraffin blocks.</p><p>Fetus sections were stained with Hematoxylin§Eosin and Cresyl-Echt Violet for general morphological observation and with SHH, SOX2, SOX10, Nestin and p75 markers for immunohistochemical findings. As a result of these stainings, morphological data were obtained about the stem cell character and migration routes in the fetus, depending on the increase and decrease in expression and the location of these alterations. In order to support these data with the amount of protein, ELISA tests were performed with SHH, SOX2 and TNGFR markers.</p><p>When we examine the literature, it is seen that the opinion that the new</p><p>generation anti-epileptic drugs are safe dominates, but with the idea that these studies should be detailed, two kind doses, below normal and very high, were selected by using specific markers and methods. The purpose of choosing a subnormal dose is to see an effect close to almost never used in a safe agent, and in choosing a very high normal dose, the aim is to keep the toxicity high in order to observe possible damage, while forming a model for extreme cases. Findings for low doses revealed that the effects of anti-epileptic drugs are almost as safe as they have never been used, in line with the literature, and some pathological changes and migratory disorders can be observed in overdoses. It has been tried to understand the migration mechanisms by examining the developmental differences seen in overdose.</p><p>As a result, it was determined that the drug is as safe as in the literature, and it showed that further studies are required for the results to be observed only with the disruption of the migratory pathways at excessively high doses.</p>