Optimization of Biotransformation of Astragaloside VII to Astragaloside IV by Immobilization of β-Glucosidase Enzyme on Magnetic Nanoparticles

Project no. 31916 · Status: Kapanmış (Yayın bekleniyor) · 2024

Summary

<p class="ql-align-justify">Astragaloside&nbsp;IV&nbsp;is&nbsp;a&nbsp;saponin-type&nbsp;secondary&nbsp;metabolite&nbsp;that&nbsp;can&nbsp;also&nbsp;be&nbsp;produced&nbsp;from&nbsp;its&nbsp;<span style="color: rgb(32, 33, 36);">glycosylic</span><span style="color: red;">&nbsp;</span>derivatives&nbsp;by&nbsp;extraction,&nbsp;separation,&nbsp;microbial&nbsp;and&nbsp;enzymatic&nbsp;hydrolysis.&nbsp;Astragaloside&nbsp;IV&nbsp;can&nbsp;be&nbsp;obtained&nbsp;as&nbsp;a&nbsp;result&nbsp;of&nbsp;hydrolysis&nbsp;of&nbsp;Astragaloside&nbsp;VII&nbsp;which&nbsp;is&nbsp;extracted&nbsp;from&nbsp;Radix&nbsp;Astragali.&nbsp;The&nbsp;chemical&nbsp;processes&nbsp;used&nbsp;for&nbsp;the&nbsp;production&nbsp;of&nbsp;Astragaloside&nbsp;IV&nbsp;lead&nbsp;to&nbsp;the&nbsp;formation&nbsp;of&nbsp;many&nbsp;by-products&nbsp;and&nbsp;chemical&nbsp;waste&nbsp;that&nbsp;are&nbsp;difficult&nbsp;to&nbsp;remove.&nbsp;Alternative&nbsp;biological&nbsp;hydrolysis&nbsp;methods&nbsp;are&nbsp;being&nbsp;developed&nbsp;to&nbsp;solve&nbsp;this&nbsp;problem.&nbsp;Reuse&nbsp;of&nbsp;enzymes&nbsp;by&nbsp;immobilization&nbsp;without&nbsp;a&nbsp;significant&nbsp;decrease&nbsp;in&nbsp;their&nbsp;activity&nbsp;is&nbsp;important&nbsp;because&nbsp;of&nbsp;advantages&nbsp;such&nbsp;as&nbsp;process&nbsp;continuity,&nbsp;ease&nbsp;of&nbsp;downstream&nbsp;processing,&nbsp;and&nbsp;pollution&nbsp;reduction.&nbsp;Although&nbsp;in&nbsp;bioprocesses&nbsp;the&nbsp;use&nbsp;of&nbsp;enzymes&nbsp;in&nbsp;free&nbsp;form&nbsp;is&nbsp;still&nbsp;common&nbsp;today,&nbsp;it&nbsp;doesn’t&nbsp;allow&nbsp;the&nbsp;reuse&nbsp;of&nbsp;enzymes,&nbsp;which&nbsp;increases&nbsp;the&nbsp;cost.&nbsp;Making&nbsp;enzymes&nbsp;reusable&nbsp;through&nbsp;the&nbsp;immobilization&nbsp;process,&nbsp;can&nbsp;significantly&nbsp;reduce&nbsp;the&nbsp;cost.&nbsp;Because&nbsp;it&nbsp;provides&nbsp;the&nbsp;opportunity&nbsp;to&nbsp;separate&nbsp;enzymes&nbsp;from&nbsp;the&nbsp;hydrolysis&nbsp;medium&nbsp;by&nbsp;simple&nbsp;magnetic&nbsp;effect,&nbsp;the&nbsp;use&nbsp;of&nbsp;magnetic&nbsp;nanoparticles&nbsp;(MNP)&nbsp;in&nbsp;enzyme&nbsp;immobilization&nbsp;provides&nbsp;time&nbsp;and&nbsp;cost&nbsp;savings.&nbsp;In&nbsp;the&nbsp;project&nbsp;titled&nbsp;"<strong>Usage&nbsp;of&nbsp;magnetic&nbsp;nanoparticle&nbsp;immobilized&nbsp;beta&nbsp;glucosidase&nbsp;and&nbsp;beta&nbsp;xylosidase&nbsp;enzymes&nbsp;in&nbsp;biotransformation&nbsp;of&nbsp;prodrug&nbsp;molecules''</strong>,&nbsp;which&nbsp;was&nbsp;previously&nbsp;completed&nbsp;by&nbsp;our&nbsp;team,&nbsp;the&nbsp;β&nbsp;Glucosidase&nbsp;(<em>Aspergillus&nbsp;niger</em>)&nbsp;enzyme&nbsp;Fe<sub>3</sub>O<sub>4</sub>&nbsp;was&nbsp;immobilized&nbsp;on&nbsp;magnetic&nbsp;nanoparticles&nbsp;(MNP),&nbsp;and&nbsp;hydrolysis&nbsp;of&nbsp;Astragaloside&nbsp;VII&nbsp;to&nbsp;Astragaloside&nbsp;IV&nbsp;has&nbsp;also&nbsp;been&nbsp;successfully&nbsp;performed.&nbsp;In&nbsp;this&nbsp;proposed&nbsp;project,&nbsp;it&nbsp;is&nbsp;aimed&nbsp;to&nbsp;make&nbsp;changes&nbsp;or&nbsp;optimizations&nbsp;for&nbsp;the&nbsp;factors&nbsp;that&nbsp;can&nbsp;be&nbsp;effective&nbsp;in&nbsp;increasing&nbsp;the&nbsp;performance&nbsp;of&nbsp;the&nbsp;hydrolysis&nbsp;reaction,&nbsp;based&nbsp;on&nbsp;the&nbsp;previous&nbsp;results.</p><p class="ql-align-center"></p><p class="ql-align-center"></p>

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