EFFECT OF PROPOLIS ON VOLTAGE GATED SODIUM CHANNELS IN COLON CANCER CELLS
Project no. 20681 · Status: Kapanmış (Yayın bekleniyor) · 2019
Summary
In vivo and in vitro studies have shown that increased expression of voltage-gated sodium channels (VGSC) in many cancers (such as breast, cervix, colon, prostate) is associated with tumor growth. VGSC stimulates many cell behaviors involved in metastasis, including motility, secretion, adhesion, and invasion. Therefore, control of VGSC activity has become an important target in cancer treatment and prevention of metastasis. It is recommended to use natural products rather than drugs for the inhibition of VGSC. Propolis, a natural product produced by honey bees, has antioxidant, anti-inflammatory and carcinostatic effects with its phenolic compounds. In our TUBITAK project, it has been shown that propolis induces apoptosis in cancer cell lines and is not cytotoxic in macrophage cells or normal cells, and it seems to be a natural product that can be used safely to prevent the spread of colon cancer. The aim of this project is to investigate the effect of propolis on VGSC and related pathways. For this purpose, measurements was made by adding propolis at the doses determined in our previous TUBITAK project in colon cancer cell culture. In this study, it was planned to determine the gene expression levels of VGSC (Nav 1.5) in colon cancer cell culture, as well as to determine the levels of INSIG2 (Insulin Induced Gene 2), which has been shown to be strongly correlated with VGSC, and PPP2R1A (Protein phosphatase 2A subunit), which has been shown to be the most important in the regulation of Wnt signal category.After the propolis samples were prepared in the Department of Food Engineering at Ege University, the phenolic content was determined in the Department of Medical Biochemistry of the Faculty of Medicine. The cell culture study was carried out in the Imperial College Molecular Biology laboratory. PP2R1A levels were found to be low in cancer cells. It was determined that PP2R1A levels did not change in healthy cells with the application of propolis, but increased in cancer cells. Our results revealed that PP2R1A levels, which are important in tumor invasion and one of the target molecules in cancer treatment, can be increased with propolis, giving hope for the use of propolis in the treatment of colon cancer.