Effects of lncRNA PVT1/miR145-5p/ABCC-1 gene line in hepatocellular carcinoma cells on cancer mechanism
Project no. 31891 · Status: Kapanmış (Yayın bekleniyor) · 2024
Summary
<p>Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and a leading cause of cancer-related mortality worldwide. Long non-coding RNAs (lncRNAs) are defined as a group of RNAs longer than 200 bp that do not encode proteins. LncRNAs play crucial regulatory roles in transcription and post-transcriptional processes. Aberrant expression of lncRNAs has been linked to numerous human diseases, including cancer. Among these, lncRNA PVT1 is a well-characterized long non-coding RNA with critical functions in various cancer types. Like other lncRNAs, PVT1 can act as a molecular sponge for microRNAs, including miR-145-5p. miR-145-5p is a key regulator of apoptosis, cell cycle progression, and cell migration. Additionally, miR-145-5p plays a pivotal role in chemoresistant malignant cells by targeting the ABCC1 gene. In this study, we aim to analyze the effects of lncRNA PVT1 knockdown on the miR-145-5p/ABCC1 axis and to elucidate the biological impact of PVT1 and miR-145-5p on hepatocellular carcinoma cells. To achieve this, we will investigate the lncRNA PVT1/miR-145-5p/ABCC1 axis in the HepG2 cell line, examining how alterations in their expression influence malignant liver cell characteristics. First, we plan to assess how PVT1 silencing affects miR-145-5p and ABCC1 expression levels in HCC cells. Furthermore, we will analyze the functional consequences of PVT1, miR-145-5p, and ABCC1 expression changes on apoptosis, cell cycle progression, metastatic potential, and the response of HepG2 cells to doxorubicin treatment.</p>