VİBURNUM OPULUS L. EKSTRELERİNİN ÜROPATOJENİK PROTEUS MİRABİLİS İZOLATLARI ÜZERİNE ANTİBAKTERİYEL ETKİLERİNİN ARAŞTIRILMASI

Proje no. 301 · Durum: Kapanmış · 2018

Özet

In this thesis, it was aimed to investigate the antibacterial and antivirulence effects of Viburnum opulus L. fruit extracts on Proteus mirabilis, which is an important cause of catheter-related urinary tract infections. This is the clinical P. mirabilis isolates used in Ege University Medical Faculty Medical Microbiology Department Bacteriology Laboratory and P. mirabilis HI4320 obtained from Mobley Research (Ann Arbor, United States) in reference, V. opulus. Minimal inhibitory concentration of V. opulus L. water, methanol and n-butanol extracts in P. mirabilis HI4320 reference strain. The effects of swarming action, urease ezyme inhibition and hemolysis in reference strains of these extracts were investigated.Among the tested extracts, water and methanol extracts of V. opulus L. showed no antibacterial effect against P. mirabilis isolates, while n-butanol extract showed weak antibacterial effect against P. mirabilis isolates. After 24 hours of incubation, V. opulus L. water and methanol extracts did not have an inhibitory effect on the swarming movement of P. mirabilis, while n-butanol extract showed a weak inhibitory effect on the swarming movement. None of the water, methanol and n-butanol extracts showed any inhibitory effect on urease enzyme even at 1/10 concentration. Water, methanol and n-butanol extracts also had no hemolysin inhibition effect at this concentration.The fact that the studied extracts did not show significant antibacterial effect on P. mirabilis clinical and reference strains is attributed to the fact that these extracts do not contain sufficient amounts of phenolic compounds and the concentrations studied were insufficient for antibacterial effect. In future studies, more pronounced antibacterial effect may be observed by selecting genotypes of V. opulus L. fruits rich in phenolic compounds. In addition, since the leaves, branches and seeds of the V. opulus L. plant contain higher levels of phenolic substances than the fruits, more antibacterial effects may be found in future studies using these parts. However, the use of phenolic compounds contained in this fruit as an antibacterial agent in studies may yield more significant results than the use of fruit extracts.

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