ENCAPSULATION OF HUMAN CORD BLOOD-DERIVED PLATELET LYSATE IN NANOVESICULAR SYSTEMS AND INVESTIGATION OF ITS WOUND HEALING POTENTIAL
Project no. 511 · Status: Kapanmış · 2018
Summary
Niosomes, being one of the nano vesicular systems, are getting attention with the latest developments in nanotechnology and are being used in various fields including wound healing in medicine. On the other hand, the efficiency of the growth factors in platelet alpha granules on wound healing is shown in numerous studies. In this doctorate study, platelets obtained from cord blood, which is considerable as biological waste after birth, are converted to lysates with independent granule content and these platelet lysates are encapsulated to niosomes with more than 80% high encapsulation efficiency. With the characterization studies, particle size analyses are made with DLS and AFM, as a result, their sizes are recorded approximately as 150 nm. The wound healing study carried out with the Human Keratinocyte Cell Line resulted a significant difference between the control group and NiPLs, on the other hand, there is no significant difference between the PL and NiPLs is observed, as expected. Besides, cell uptake studies show that NiPLs can easily be taken into the cell and accumulated in cytoplasm. PDGF-AB dependent short term stability test carried out with the Human PDGF-AB ELISA. This experiment shows that NiPLs are significantly more stable than free PLs. These results show NiPLs has a great potential to be used in wound healing with their high stabilities.