Comparative Study Of The Effects Of Subcutaneously Administered Umbilical Cord-Derived Mesenchymal Stem Cells And Exosomes Derivatives On Wound Healing In A Chemical Burn Model Of Alkaline-Induced Skin Burns
Project no. 32603 · Status: Kapanmış · 2024
Summary
<p><strong>Objective: </strong>The primary goal in burn-injured skin is to accelerate wound healing and restore the disrupted barrier. In chemical burns, excessive inflammation and deep tissue damage are major challenges. This study aimed to investigate the effects of human umbilical cord-derived mesenchymal stem cells (HucMSCs) and their exosomes (Exo-HucMSCs) on wound healing in a chemical burn model.</p><p><strong>Materials and Methods: </strong>A chemical burn was induced on the dorsal skin of 24 Wistar Albino rats using 2 M sodium hydroxide. Rats were randomly assigned to three groups (n = 8): HucMSC-treated, Exo-HucMSC-treated, and control. Wound closure rates and histopathological changes were evaluated and compared among groups.</p><p><strong>Results: </strong>Wound closure was significantly higher in the HucMSC group (78.15% ± 9.19) than in the Exo-HucMSC (66.11% ± 7.33) and control groups (57.35% ± 4.54) (p < 0.05). Histologically, treatment groups showed early inflammatory changes on days 1 and 3 (p < 0.01), enhanced striated muscle regeneration on day 7 (p < 0.01), and narrower non-epithelialized areas on day 21 (p < 0.05). Neoangiogenesis was significantly greater in the Exo-HucMSC group on day 3 (p < 0.01).</p><p><strong>Conclusion: </strong>Both HucMSCs and Exo-HucMSCs applications accelerated the chemical burn healing process. Exosomes promoted early-phase angiogenesis, while HucMSCs provided superior long-term wound closure.</p>