DESIGN AND APPLICATIONS OF ELECTROCHEMICAL BIOSENSORS FOR THE DETECTION OF PLANT EXTRACT-DNA INTERACTIONS
Project no. 275 · Status: Kapanmış · 2016
Summary
In this Project, single use electrochemical DNA biosensors were developed for direct detection of possible interactions between DNA and compounds in plant extracts. The calf thymus double-stranded DNA (dsDNA) and synthetic probe / target DNA sequences were interacted with plant extracts of Sempervivum davisii Muirhead, Plantago anatolica Tutel & R. Mill with. and Verbascum cheiranthifolium Boiss. var. Cheiranthifolium. The interactions of plant extracts were investigated by differential pulse voltammetry (DPV) technique based on guanine and extract oxidation signals by using pencil graphite electrode (KGE). All three plant extracts were found to interact with DNA. Changes in experimental parameters, such as extract concentration or time to extract-DNA interactions, were investigated using response differences in both guanine and plant extract electroactivity. Additionally, the application of electrochemical methods for the detection of interactions between DNA and DNA-targeted any kind of substances was investigated and discussed. The obtained results are also promising for practical applications in the analysis of compounds that cause DNA damage. Keywords: Electrochemistry, DNA, biosensor, plant extract and DNA interaction, Sempervivum davisii Muirhead, Plantago anatolica Tutel & R. Mill. ve Verbascum cheiranthifolium Boiss. var. cheiranthifolium Boiss.