Investigation Of the Effects of Autophagy On Fat Body Metabolism During Larval-Pupal Metamorphosis Of Silkworm Bombyx mori
Project no. 23535 · Status: Kapanmış · 2022
Summary
<p>Adipose tissue is a large tissue distributed throughout the body of the silkworm. Analyses of metabolic processes during insect development are often carried out in adipose tissue because it plays an important role in storage, immunity, management of energy reserves, reproduction and development. Autophagy, an important cellular mechanism, plays a key role in cell survival under stress conditions such as starvation, as well as in the elimination of redundant or damaged structures and organelles, and in the onset and regulation of cell death.</p><p>The aim of this study was to investigate the role of autophagy in adipose tissue metabolism of the silkworm Bombyx mori during larval-pupal metamorphosis using the autophagy inhibitor chloroquine. Treatment and control silkworms were photographed, weighed and adipose tissue samples obtained on the determined days were analyzed morphologically and histochemically. In addition, the glycogen, sugar, lipid and protein contents of the adipose tissue samples were determined spectrophotometrically. The level of autophagy was determined by measuring acid phosphatase activity. Atg8 protein was determined by Western blot and analyzed as an autophagy marker. The results obtained showed that inhibition of autophagy mechanism by chloroquine application disrupted the energy metabolism of insects in general and prevented the metamorphosis process.</p>