Investigation of lipase production and oil biodegradation potentials of actinobacteria isolated from different environments
Project no. 21960 · Status: Kapanmış (Yayın bekleniyor) · 2020
Summary
<p>Lipids are important organic substances and can cause serious environmental pollution in some industrial wastewaters. Today, with industrialization, there is an increasing lipid accumulation in wastewater. Reducing this accumulation can be achieved with the lipase enzyme.</p><p><br></p><p> Lipases are enzymes capable of catalyzing the hydrolysis of esters from glycerol and long chain fatty acids. These enzymes are derived from microorganisms that produce a wide variety of extracellular lipases.</p><p><br></p><p> Lipases are inducible enzyme used in the purification of oil-rich residues and require some oil, fatty acid, fatty acid alcohol or fatty acid ester and surfactant for induction. The bioconversion feature of the lipase enzyme is seen as a safer waste treatment option that will solve environmental problems, and it is known today that the use of microbial enzymes greatly reduces the production cost.</p><p><br></p><p> In the light of this information, in our study, actinobacteria, which are lipase enzyme producers, will be selected by screening the lipolytic activities of actinobacteria isolates obtained from marine and terrestrial environments in terms of lipase enzyme production properties.</p><p><br></p><p> Selected isolates will be screened for lipase enzyme production activity and the isolate with the highest lipase activity will be determined. By changing various nutrient media contents such as carbon and nitrogen sources in this isolate production environment, the production will be carried out by choosing the most suitable environment in terms of enzyme production and activity, where the highest enzyme synthesis takes place, and the potential for oil biodegradation in the wastewater will be investigated.</p>